Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,096,745	C→T	S235N (AGC→AAC)	rhaB ←	rhamnulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,096,745	0	C	T	100.0%	95.8 / NA	30	S235N (AGC→AAC)	rhaB	rhamnulokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/16);  total (14/16)

CTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTC  >  NC_000913/4096614‑4096881                                                                                                                                    |                                                                                                                                         cTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCaa                                                                                                                                    >  2:368396/1‑138 (MQ=255) cTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCaa                                                                                                                                    <  1:368396/138‑1 (MQ=255) cTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACg                                                                                                                                  >  1:308664/1‑140 (MQ=255)  tGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACgg                                                                                                                                 >  2:322721/1‑140 (MQ=255)     cAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGa                                                                                                                              <  1:125136/140‑1 (MQ=255)     cAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGa                                                                                                                              <  2:107044/140‑1 (MQ=255)     cAGTGCCGTGTCATTGGTAAATGGCGTCTGACTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGa                                                                                                                              <  1:209459/140‑1 (MQ=255)                      tAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTa                                                                                                             <  2:480486/140‑1 (MQ=255)                               tCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCggg                                                                                                    >  1:112713/1‑140 (MQ=255)                               tCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCggg                                                                                                    >  1:372245/1‑140 (MQ=255)                               tCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCggg                                                                                                    >  1:98161/1‑140 (MQ=255)                                 tGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCa                                                                                                  <  2:98161/140‑1 (MQ=255)                                     tttCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGCTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATc                                                                                              <  2:258400/140‑1 (MQ=255)                                        cGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAg                                                                                           <  1:34773/140‑1 (MQ=255)                                         gAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGt                                                                                          <  2:504123/140‑1 (MQ=255)                                            gCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGAc                                                                                       <  1:322721/140‑1 (MQ=255)                                                 tCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCaa                                                                                                 <  1:242809/125‑1 (MQ=255)                                                 tCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCaa                                                                                                 >  2:242809/1‑125 (MQ=255)                                                               ccAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATg                                                                    >  2:226566/1‑140 (MQ=255)                                                                 agaagaGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGGATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCg                                                                  >  2:156824/1‑140 (MQ=255)                                                                             agcagcACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCaa                                                      >  2:202968/1‑140 (MQ=255)                                                                               cagcaCGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAAc                                                    >  2:377555/1‑140 (MQ=255)                                                                                  caCGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAg                                                 >  2:188359/1‑140 (MQ=255)                                                                                                    gcgaggcgaTAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTc                               <  2:372245/140‑1 (MQ=255)                                                                                                       aggcgaTAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCAc                            >  1:311073/1‑140 (MQ=255)                                                                                                         gcgaTAACCGCGCTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGc                          <  1:487615/140‑1 (MQ=255)                                                                                                                   cgcTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAg                    <  2:472575/136‑1 (MQ=255)                                                                                                                   cgcTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAg                    >  1:472575/1‑136 (MQ=255)                                                                                                                   cgcTGGCGGTATCATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGAc                >  1:263865/1‑140 (MQ=255)                                                                                                                               cATGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAgtcg    <  2:263865/140‑1 (MQ=255)                                                                                                                                aTGGTTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAgtcgt   <  2:112713/140‑1 (MQ=255)                                                                                                                                 tggttggcAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAgtcgtc  <  1:377555/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         CTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTC  >  NC_000913/4096614‑4096881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑