Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,204,531	A→G	E404E (GAA→GAG)	zraR →	DNA‑binding transcriptional activator ZraR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,204,531	0	A	G	100.0%	64.5 / NA	19	E404E (GAA→GAG)	zraR	DNA‑binding transcriptional activator ZraR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/12);  total (7/12)

GCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAG  >  NC_000913/4204399‑4204664                                                                                                                                     |                                                                                                                                      gCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGaaa                                                                                                                                <  1:418987/140‑1 (MQ=255)                ggAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTgg                                                                                                                 <  2:100558/140‑1 (MQ=255)                 gAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTggc                                                                                                                >  2:393249/1‑140 (MQ=255)                 gAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTggc                                                                                                                >  2:215419/1‑140 (MQ=255)                  aaCGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTggcg                                                                                                               <  2:185088/140‑1 (MQ=255)                            ggtgCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGgaga                                                                                                     >  2:134181/1‑140 (MQ=255)                                ctgctgACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAAc                                                                                                 >  2:182604/1‑140 (MQ=255)                                          gggAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGcaac                                                                                       >  1:164652/1‑140 (MQ=255)                                                         aaCGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAgccgcc                                                                        >  2:172679/1‑140 (MQ=255)                                                           cgcgAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCg                                                                      >  2:7470/1‑140 (MQ=255)                                                            gcgAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGt                                                                     <  1:172679/140‑1 (MQ=255)                                                            gcgAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGt                                                                     <  2:228083/140‑1 (MQ=255)                                                            gcgAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGt                                                                     <  2:164652/140‑1 (MQ=255)                                                            gcgAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGt                                                                     <  1:182604/140‑1 (MQ=255)                                                                        tGGCGATTACCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATc                                                         <  2:39978/140‑1 (MQ=255)                                                                          gCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCAc                                                       <  1:393249/140‑1 (MQ=255)                                                                          gCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCAc                                                       <  1:215419/140‑1 (MQ=255)                                                                                      aCGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCt                                           <  1:7470/140‑1 (MQ=255)                                                                                                    gggACAAAGTCAGGATATTCAGCCGTTGGTGGAGGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGt                             <  1:351986/140‑1 (MQ=255)                                                                                                                               ggtggaggtggaAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGCTCTGCTCGCGTTCGATAg  >  2:155045/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      GCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAG  >  NC_000913/4204399‑4204664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑