Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,291,652	C→T	S47S (AGC→AGT)	nrfE →	putative cytochrome c‑type biogenesis protein NrfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,291,652	0	C	T	100.0%	84.4 / NA	25	S47S (AGC→AGT)	nrfE	putative cytochrome c‑type biogenesis protein NrfE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

CCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGGGCGGCCATGAAGGTTCGCTGCTGCTTTGGGTGCTGCTGCTTTCCGCCTGGAGCGCGCT  >  NC_000913/4291519‑4291786                                                                                                                                      |                                                                                                                                       ccccGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTtc                                                                                                                                  <  2:526301/140‑1 (MQ=255) ccccGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTtc                                                                                                                                  >  2:217677/1‑140 (MQ=255)    cGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCg                                                                                                                               <  1:55020/140‑1 (MQ=255)    cGTTGACGGCCTTCCCGGGCGCGCGGTTACACTGGCCTGCCACGGTGGGACTCACCTCCATCGGCATTCTGGCGCAGGTCCCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGGATTGTTTTCTCATCACGGATTTTTCg                                                                                                                               >  2:454056/1‑140 (MQ=255)          cGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCAtt                                                                                                                         >  1:258397/1‑140 (MQ=255)                tCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTc                                                                                                                   >  2:233529/1‑140 (MQ=255)                tCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTc                                                                                                                   >  1:509863/1‑140 (MQ=255)                   cgGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTcgcg                                                                                                                <  1:132832/140‑1 (MQ=255)                   cgGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTcgcg                                                                                                                <  1:447463/140‑1 (MQ=255)                         tGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCgcgc                                                                                                               >  1:466429/1‑135 (MQ=255)                         tGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCgcgc                                                                                                               <  2:466429/135‑1 (MQ=255)                                 cgcTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGCCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCACATCAGTGATTTCTCGGTCATTTACGTCGCGCAACATAGCTATAg                                                                                                  <  2:505355/140‑1 (MQ=255)                                      gCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCctg                                                                                              >  2:371904/1‑139 (MQ=255)                                      gCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCctg                                                                                              <  1:371904/139‑1 (MQ=255)                                                                   gCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGgtg                                                                >  2:76310/1‑140 (MQ=255)                                                                            cgcAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCa                                                                      >  2:478398/1‑125 (MQ=255)                                                                            cgcAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCa                                                                      <  1:478398/125‑1 (MQ=255)                                                                            cgcAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGggcggc                                                       >  2:87249/1‑140 (MQ=255)                                                                                          ctgctgCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGGGCGGCCATGAAGGTTCgct                                         <  1:217677/140‑1 (MQ=255)                                                                                                    ccTTTGGCGTACTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGGGCGGCCATGAAGGTTCGCTGCTGCTTTgg                               >  1:436660/1‑140 (MQ=255)                                                                                                              aCTGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGGGCGGCCATGAAGGTTCGCTGCTGCTTTGGGtgctgctgc                     <  1:87249/140‑1 (MQ=255)                                                                                                                tGACGTATTGTTTTCTCATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGGGCGGCCATGAAGGTTCGCTGCTGCTTTGGGTGCTGCTGCtt                   <  1:105414/140‑1 (MQ=255)                                                                                                                             tctcATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGGGCTGCCATGAAGGTTCGCTGCTGCTTTGGGTGCTGCTGCTTTCCGCCTGGAgcg      <  2:132551/140‑1 (MQ=255)                                                                                                                             tctcATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGGGCGGCCATGAAGGTTCGCTGCTGCTTTGGGTGCTGCTGCTTTCCGCCTGGAgcg      <  2:255249/140‑1 (MQ=255)                                                                                                                             tctcATCAGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGGGCGGCCATGAAGGTTCGCTGCTGCTTTGGGTGCTGCTGCTTTCCGCCTGGAgcg      <  1:233529/140‑1 (MQ=255)                                                                                                                                 atcaGTGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGGGCGGCCATGAAGGTTCGCTGCTGCTTTGGGTGCTGCTGCTTTCCGCCTGGAGCGCGCt  >  2:133577/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       CCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGATTTCTCGGTCATTTATGTCGCGCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAGCTGGCAGCGGTGTGGGGCGGCCATGAAGGTTCGCTGCTGCTTTGGGTGCTGCTGCTTTCCGCCTGGAGCGCGCT  >  NC_000913/4291519‑4291786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑