Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,413,019	A→G	G211G (GGA→GGG)	yjfM →	DUF1190 domain‑containing protein YjfM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,413,019	0	A	G	100.0%	85.6 / NA	24	G211G (GGA→GGG)	yjfM	DUF1190 domain‑containing protein YjfM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/13);  total (11/13)

GATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTT  >  NC_000913/4412891‑4413145                                                                                                                                 |                                                                                                                               gATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATg                                                                                                                     >  2:42503/1‑140 (MQ=255)   ttACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCt                                                                                                                   <  2:207483/140‑1 (MQ=255)       tCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTgaga                                                                                                               <  2:457136/140‑1 (MQ=255)       tCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTgaga                                                                                                               >  1:457136/1‑140 (MQ=255)                 ccGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTc                                                                                                     >  1:417861/1‑140 (MQ=255)                 ccGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTc                                                                                                     >  2:374391/1‑140 (MQ=255)                          gCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGAc                                                                                            <  2:417861/140‑1 (MQ=255)                           cAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACg                                                                                           <  1:42503/140‑1 (MQ=255)                               aaaGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGAt                                                                                       <  1:156885/140‑1 (MQ=255)                                aaGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATc                                                                                      <  1:259054/140‑1 (MQ=255)                                        tACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAg                                                                              >  1:256289/1‑140 (MQ=255)                                         aCTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGa                                                                             >  1:428068/1‑140 (MQ=255)                                                        ttACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTAGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACg                                                              <  1:477519/140‑1 (MQ=255)                                                                aaaaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGAc                                                      >  1:139050/1‑140 (MQ=255)                                                                aaaaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGAc                                                      <  1:374391/140‑1 (MQ=255)                                                                             ccGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCg                                         <  1:350098/140‑1 (MQ=255)                                                                                            gcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAtt                          <  1:115486/140‑1 (MQ=255)                                                                                                      ttcttcCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGagag                >  2:271268/1‑140 (MQ=255)                                                                                                      ttcttcCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGagag                >  2:78771/1‑140 (MQ=255)                                                                                                        cttcCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAt                     <  1:433271/133‑1 (MQ=255)                                                                                                        cttcCAGCGCCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAt                     >  2:433271/1‑133 (MQ=255)                                                                                                               cgcCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTa       <  1:78771/140‑1 (MQ=255)                                                                                                               cgcCCGTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTa       >  2:47607/1‑140 (MQ=255)                                                                                                                    gTGGGCATTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGtt  <  2:139050/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                               GATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTT  >  NC_000913/4412891‑4413145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑