Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,444,316	G→A	G69S (GGC→AGC)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,444,316	0	G	A	100.0%	91.4 / NA	27	G69S (GGC→AGC)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

GGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCC  >  NC_000913/4444202‑4444443                                                                                                                   |                                                                                                                                ggCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGGAAAAACgggggatat                                                                                                        >  2:267474/1‑134 (MQ=255)            ggTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTg                                                                                             <  2:309714/140‑1 (MQ=255)             gTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGt                                                                                            <  2:45481/140‑1 (MQ=255)                   aaaGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCt                                                                                      >  1:486840/1‑140 (MQ=255)                       cggcggATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAg                                                                                  >  1:221428/1‑140 (MQ=255)                        ggcggATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGt                                                                                 >  1:166195/1‑140 (MQ=255)                          cggATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGc                                                                               <  2:384977/140‑1 (MQ=255)                          cggATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGc                                                                               >  1:384977/1‑140 (MQ=255)                          cggATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGc                                                                               >  1:365336/1‑140 (MQ=255)                               cGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCtgtg                                                                          <  2:221428/140‑1 (MQ=255)                                     gTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAAcag                                                                    <  1:4576/140‑1 (MQ=255)                                       gCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAAcagca                                                                  <  2:166195/140‑1 (MQ=255)                                           ggACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAgtgt                                                              <  1:422764/140‑1 (MQ=255)                                                       ggCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGa                                                  >  1:292737/1‑140 (MQ=255)                                                                  cggcggCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGa                                       >  1:59197/1‑140 (MQ=255)                                                                  cggcggCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGa                                       >  2:236060/1‑140 (MQ=255)                                                                              cgATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAgg                           <  1:290285/140‑1 (MQ=255)                                                                              cgATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAgg                           <  1:267474/140‑1 (MQ=255)                                                                              cgATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAgg                           <  1:236060/140‑1 (MQ=255)                                                                                     aCCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAgg                           >  1:453073/1‑133 (MQ=255)                                                                                     aCCTTGTCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAgg                           <  2:453073/133‑1 (MQ=255)                                                                                           tCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACAtt                               <  2:502240/123‑1 (MQ=255)                                                                                           tCTGACGTTCGTTATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACAtt                               >  1:502240/1‑123 (MQ=255)                                                                                                    cgttATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAg              <  2:442491/131‑1 (MQ=255)                                                                                                    cgttATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAg              >  1:442491/1‑131 (MQ=255)                                                                                                    cgttATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAAt     >  2:190736/1‑140 (MQ=255)                                                                                                       tATGAGCAGCCAAGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGcc  <  2:486840/140‑1 (MQ=255)                                                                                                                   |                                                                                                                                GGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTGTTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCC  >  NC_000913/4444202‑4444443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑