Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,444,616	A→G	T169A (ACC→GCC)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,444,616	0	A	G	100.0%	122.8 / NA	34	T169A (ACC→GCC)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

GCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAA  >  NC_000913/4444483‑4444749                                                                                                                                      |                                                                                                                                      gCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAAccctggccctga                                                                                                                                 >  1:396523/1‑140 (MQ=255)                 ccGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGaa                                                                                                                 >  1:131876/1‑140 (MQ=255)                     aTCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGc                                                                                                             >  1:133416/1‑140 (MQ=255)                     aTCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGc                                                                                                             >  2:350722/1‑140 (MQ=255)                               ccTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTg                                                                                                   <  2:271927/140‑1 (MQ=255)                                 tGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGct                                                                                                 >  1:138977/1‑140 (MQ=255)                                 tGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGct                                                                                                 >  1:151756/1‑140 (MQ=255)                                 tGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGct                                                                                                 >  1:207560/1‑140 (MQ=255)                                 tGACACGGGTGGCACAGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGct                                                                                                 >  2:479259/1‑140 (MQ=255)                                         gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGct                                                                                                 >  2:390842/1‑132 (MQ=255)                                         gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGct                                                                                                 <  1:390842/132‑1 (MQ=255)                                         gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGaa                                                                                         >  1:375874/1‑140 (MQ=255)                                         gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGaa                                                                                         >  2:338489/1‑140 (MQ=255)                                         gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGaa                                                                                         <  2:355/140‑1 (MQ=255)                                         gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGaa                                                                                         <  1:350722/140‑1 (MQ=255)                                           ggCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGaa                                                                                         <  1:239878/138‑1 (MQ=255)                                           ggCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGaa                                                                                         >  2:239878/1‑138 (MQ=255)                                                        gTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGgc                                                                          >  2:412091/1‑140 (MQ=255)                                                        gTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGgc                                                                          <  1:199193/140‑1 (MQ=255)                                                        gTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGgc                                                                          <  2:207560/140‑1 (MQ=255)                                                        gTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGgc                                                                          <  2:133416/140‑1 (MQ=255)                                                        gTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGgc                                                                          <  2:138977/140‑1 (MQ=255)                                                            aCATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAg                                                                      <  1:479259/140‑1 (MQ=255)                                                                  aGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGgaagaa                                                                <  1:412091/140‑1 (MQ=255)                                                                        atgaCACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTc                                                          >  1:52991/1‑140 (MQ=255)                                                                            caccacGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGGGGCGCAGGAAGAAGTGGTCGAAc                                                      >  2:315607/1‑140 (MQ=255)                                                                                            aTGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATcc                                      >  1:531791/1‑140 (MQ=255)                                                                                            aTGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATcc                                      >  1:444210/1‑140 (MQ=255)                                                                                             tGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCg                                     <  2:444210/140‑1 (MQ=255)                                                                                              ggACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGc                                    >  2:56914/1‑140 (MQ=255)                                                                                                      ccTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGAt                            <  1:338489/140‑1 (MQ=255)                                                                                                            gCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAg                      >  1:367735/1‑140 (MQ=255)                                                                                                            gCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGGGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAg                      >  1:280893/1‑140 (MQ=255)                                                                                                                       cAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGa           <  1:338559/140‑1 (MQ=255)                                                                                                                        aGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGaa          <  1:56914/140‑1 (MQ=255)                                                                                                                         ggAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGaaa         <  2:131876/140‑1 (MQ=255)                                                                                                                                accctggccctgAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGaa  >  2:165350/1‑140 (MQ=255)                                                                                                                                accctggccctgAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGaa  <  1:518994/140‑1 (MQ=255)                                                                                                                                accctggccctgAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGaa  <  2:396523/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      GCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAA  >  NC_000913/4444483‑4444749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑