Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,444,672	A→G	E187E (GAA→GAG)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,444,672	0	A	G	100.0%	111.8 / NA	33	E187E (GAA→GAG)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/18);  total (15/18)

GTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGCCGCTTAAC  >  NC_000913/4444538‑4444807                                                                                                                                       |                                                                                                                                        gTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGgc                                                                                                                                    <  1:199193/140‑1 (MQ=255) gTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGgc                                                                                                                                    >  2:412091/1‑140 (MQ=255) gTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGgc                                                                                                                                    <  2:207560/140‑1 (MQ=255) gTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGgc                                                                                                                                    <  2:138977/140‑1 (MQ=255) gTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGgc                                                                                                                                    <  2:133416/140‑1 (MQ=255)     aCATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAg                                                                                                                                <  1:479259/140‑1 (MQ=255)           aGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGgaagaa                                                                                                                          <  1:412091/140‑1 (MQ=255)                 atgaCACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTc                                                                                                                    >  1:52991/1‑140 (MQ=255)                     caccacGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGGGGCGCAGGAAGAAGTGGTCGAAc                                                                                                                >  2:315607/1‑140 (MQ=255)                                     aTGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATcc                                                                                                >  1:444210/1‑140 (MQ=255)                                     aTGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATcc                                                                                                >  1:531791/1‑140 (MQ=255)                                      tGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCg                                                                                               <  2:444210/140‑1 (MQ=255)                                       ggACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGc                                                                                              >  2:56914/1‑140 (MQ=255)                                               ccTCCGGCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGAt                                                                                      <  1:338489/140‑1 (MQ=255)                                                     gCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAg                                                                                >  1:367735/1‑140 (MQ=255)                                                     gCCTGAACTGGCAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGGGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAg                                                                                >  1:280893/1‑140 (MQ=255)                                                                cAGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGa                                                                     <  1:338559/140‑1 (MQ=255)                                                                 aGGAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGaa                                                                    <  1:56914/140‑1 (MQ=255)                                                                  ggAGAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGaaa                                                                   <  2:131876/140‑1 (MQ=255)                                                                         accctggccctgAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGaa                                                            <  1:518994/140‑1 (MQ=255)                                                                         accctggccctgAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGaa                                                            >  2:165350/1‑140 (MQ=255)                                                                         accctggccctgAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGaa                                                            <  2:396523/140‑1 (MQ=255)                                                                             tggccctgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGAt                                                        <  1:488226/137‑1 (MQ=255)                                                                             tggccctgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGAt                                                        >  2:385326/4‑140 (MQ=255)                                                                                    gaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCtttt                                                 <  2:531791/140‑1 (MQ=255)                                                                                          gACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGcc                                           <  2:280893/140‑1 (MQ=255)                                                                                          gACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGcc                                           <  1:315607/140‑1 (MQ=255)                                                                                           aCGTTGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGGGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGccc                                          >  1:71596/1‑140 (MQ=255)                                                                                                gCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTAt                                     >  1:281516/1‑140 (MQ=255)                                                                                                gCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTAt                                     <  2:281516/140‑1 (MQ=255)                                                                                                                       tGCCGAAAGTGGCGGAGGGGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCAt              >  1:42670/1‑140 (MQ=255)                                                                                                                                   cGGAGGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGCCGCTTAAc  >  2:424955/1‑140 (MQ=255)                                                                                                                                   cGGAGGAGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGCCGTGCATTTGCCGCTTAAc  >  2:510192/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGCCGCTTAAC  >  NC_000913/4444538‑4444807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑