Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	260,332	T→C	intergenic (‑232/‑56)	phoE ← / → proB	outer membrane porin PhoE/glutamate 5‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	260,332	0	T	C	100.0%	132.0 / NA	36	intergenic (‑232/‑56)	phoE/proB	outer membrane porin PhoE/glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/15);  total (21/15)

GATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGTCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCG  >  NC_000913/260199‑260449                                                                                                                                      |                                                                                                                      gATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCt                                                                                                                 >  2:464194/1‑140 (MQ=255)  aTTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTa                                                                                                                >  2:67562/1‑140 (MQ=255)  aTTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTa                                                                                                                >  2:38801/1‑140 (MQ=255)  aTTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTa                                                                                                                >  2:263575/1‑140 (MQ=255)  aTTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTa                                                                                                                >  2:112431/1‑140 (MQ=255)    taataaTATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTaaa                                                                                                              >  2:317820/1‑140 (MQ=255)      ataataTTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAAc                                                                                                            >  1:183295/1‑140 (MQ=255)        aataTTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAAcgt                                                                                                          <  1:464194/140‑1 (MQ=255)            ttCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAAcgttcgt                                                                                                      >  2:345145/1‑140 (MQ=255)            ttCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAAcgttcgt                                                                                                      >  2:327591/1‑140 (MQ=255)            ttCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAAcgttcgt                                                                                                      >  1:511181/1‑140 (MQ=255)               ttAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTg                                                                                                   >  1:115551/1‑140 (MQ=255)                 aaaaTGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGat                                                                                                 >  1:225597/1‑140 (MQ=255)                     tgtgGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCa                                                                                             >  2:50679/1‑140 (MQ=255)                         gTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCAtttt                                                                                         >  1:309860/1‑140 (MQ=255)                               tattaAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTaa                                                                                   >  2:260202/1‑140 (MQ=255)                                      tCTGTAATAAAAGCGAAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGaa                                                                            >  1:158752/1‑140 (MQ=255)                                        tGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATg                                                                          <  2:115551/140‑1 (MQ=255)                                          taataaAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGc                                                                        <  1:38801/140‑1 (MQ=255)                                          taataaAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGc                                                                        <  1:263575/140‑1 (MQ=255)                                             taaAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCaga                                                                     <  1:112431/140‑1 (MQ=255)                                                 aGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAAt                                                                 >  2:299999/1‑140 (MQ=255)                                                    gTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACAGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCAt                                                              >  2:178574/1‑140 (MQ=255)                                                          aacTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGa                                                        <  1:521673/140‑1 (MQ=255)                                                                    aGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACg                                              <  1:299999/140‑1 (MQ=255)                                                                      gCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCt                                            <  1:67562/140‑1 (MQ=255)                                                                      gCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCt                                            <  1:327591/140‑1 (MQ=255)                                                                             gATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTa                                     <  1:260202/140‑1 (MQ=255)                                                                                cccGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTaaaa                                  <  1:336489/140‑1 (MQ=255)                                                                                cccGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCAACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTaaaa                                  >  2:91463/1‑140 (MQ=255)                                                                                  cgcgCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACGGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACt                                <  2:182811/140‑1 (MQ=255)                                                                                    cgcAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCg                              >  2:204501/1‑140 (MQ=255)                                                                                         caaAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCAACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCAcc                         <  1:91463/140‑1 (MQ=255)                                                                                                 cATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCt                 <  2:309860/140‑1 (MQ=255)                                                                                                          cTCGCAGATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCgg        >  2:286240/1‑140 (MQ=255)                                                                                                                gATGGTTGGCAACCGACGACAGCCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATcgcg  <  1:286240/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                      GATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGTCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCG  >  NC_000913/260199‑260449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑