Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,476,836	T→C	L467P (CTA→CCA)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,476,836	0	T	C	100.0%	184.3 / NA	50	L467P (CTA→CCA)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/27);  total (23/27)

ACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCTATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACTTAAGAGTTATG  >  NC_000913/4476700‑4476970                                                                                                                                         |                                                                                                                                       aCATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCAtt                                                                                                                                     <  1:339617/140‑1 (MQ=255)      attTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATa                                                                                                                                <  2:459880/140‑1 (MQ=255)          ttCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGt                                                                                                                            <  2:353908/140‑1 (MQ=255)           tCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGtt                                                                                                                           <  1:130786/140‑1 (MQ=255)               tctcGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGt                                                                                                                       <  1:170532/140‑1 (MQ=255)               tctcGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGt                                                                                                                       <  2:274897/140‑1 (MQ=255)               tctcGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGt                                                                                                                       <  2:226750/140‑1 (MQ=255)               tctcGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGt                                                                                                                       <  1:497681/140‑1 (MQ=255)                ctcGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTc                                                                                                                      <  2:217129/140‑1 (MQ=255)                             ccTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGaa                                                                                                         >  2:387547/1‑140 (MQ=255)                              cTATAAAAATAGCCCTGAATTACAGGGGTTGATTTAGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAg                                                                                                        <  2:376350/140‑1 (MQ=255)                               tataAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATg                                                                                                           >  2:418701/1‑136 (MQ=255)                               tataAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATg                                                                                                           <  1:418701/136‑1 (MQ=255)                               tataAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGt                                                                                                       >  2:282814/1‑140 (MQ=255)                                     aaTAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGt                                                                                                 <  1:176518/140‑1 (MQ=255)                                       tAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCa                                                                                               >  2:456243/1‑140 (MQ=255)                                         gCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGc                                                                                             >  1:420534/1‑140 (MQ=255)                                          cccTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCa                                                                                            >  1:22138/1‑140 (MQ=255)                                           ccTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCAt                                                                                           >  2:331818/1‑140 (MQ=255)                                           ccTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCAt                                                                                           >  2:408090/1‑140 (MQ=255)                                               aaTTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTa                                                                                       >  1:308532/1‑140 (MQ=255)                                                    cAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGaa                                                                                  <  1:200058/140‑1 (MQ=255)                                                      ggggTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATg                                                                                <  2:230288/140‑1 (MQ=255)                                                               ttGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTa                                                                       <  1:331818/140‑1 (MQ=255)                                                                 ggAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTatg                                                                     >  1:180502/1‑140 (MQ=255)                                                                        gttggttgTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGAtt                                                              >  1:19507/1‑140 (MQ=255)                                                                           ggttgTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCt                                                           >  2:215442/1‑140 (MQ=255)                                                                                   atGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGaaa                                                   >  1:201347/1‑140 (MQ=255)                                                                                       cAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATc                                               >  1:64026/1‑140 (MQ=255)                                                                                       cAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATc                                               <  2:17102/140‑1 (MQ=255)                                                                                       cAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATc                                               >  1:501090/1‑140 (MQ=255)                                                                                         aTGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCt                                             <  1:408090/140‑1 (MQ=255)                                                                                          tGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTg                                            >  1:521543/1‑140 (MQ=255)                                                                                          tGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTg                                            >  1:271901/1‑140 (MQ=255)                                                                                          tGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTg                                            >  1:116246/1‑140 (MQ=255)                                                                                             aTTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTgaga                                         <  2:1371/140‑1 (MQ=255)                                                                                             aTTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTgaga                                         <  2:521543/140‑1 (MQ=255)                                                                                                     tAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAAt                                 <  2:201347/140‑1 (MQ=255)                                                                                                             cGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAAt                                 <  2:275837/132‑1 (MQ=255)                                                                                                             cGAGAAAAAACAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAAt                                 >  1:275837/1‑132 (MQ=255)                                                                                                                     aaCAAATTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGt                 <  1:456243/140‑1 (MQ=255)                                                                                                                         aaTTTAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACt             <  2:116246/140‑1 (MQ=255)                                                                                                                             tAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACTTAag         <  2:64026/140‑1 (MQ=255)                                                                                                                             tAATACAAAGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACTTAag         <  1:387547/140‑1 (MQ=255)                                                                                                                                    aaGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACTTa           <  2:3216/131‑1 (MQ=255)                                                                                                                                    aaGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACTTa           >  1:3216/1‑131 (MQ=255)                                                                                                                                    aaGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACTTAAGAGTTATg  <  2:308532/140‑1 (MQ=255)                                                                                                                                    aaGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACTTAAGAGTTATg  >  2:137395/1‑140 (MQ=255)                                                                                                                                    aaGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACTTAAGAGTTATg  >  1:9047/1‑140 (MQ=255)                                                                                                                                    aaGGCCATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACTTAAGAGTTATg  >  1:9046/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCTATTTGATAATGTTGAGTCTATATTTAATGAAGTACCTGTCAGCATTTTAGTGAATGATATTTTTATGAATGATTTCTTTATGAAAAATCCTGAGATGATTAATTGGTACTTCCCTCAGTTACTTAAGAGTTATG  >  NC_000913/4476700‑4476970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑