Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,593,647	A→G	V359A (GTT→GCT)	lgoT ←	galactonate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,593,647	0	A	G	100.0%	114.3 / NA	32	V359A (GTT→GCT)	lgoT	galactonate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

AGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAACCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCGGAGCCATTCCCCCTTTGACCAGCCAGTCGGTAAC  >  NC_000913/4593510‑4593777                                                                                                                                          |                                                                                                                                   agGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGcc                                                                                                                                  >  2:254012/1‑140 (MQ=255)  ggCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGccg                                                                                                                                 <  2:524717/140‑1 (MQ=255)  ggCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGccg                                                                                                                                 <  1:224010/140‑1 (MQ=255)  ggCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGccg                                                                                                                                 <  1:315105/140‑1 (MQ=255)   gcgcAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGccgc                                                                                                                                >  1:95668/1‑140 (MQ=255)    cgcAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAgccgcc                                                                                                                               <  2:95668/140‑1 (MQ=255)    cgcAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAgccgcc                                                                                                                               <  2:285452/140‑1 (MQ=255)       aGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTc                                                                                                                            <  2:43938/140‑1 (MQ=255)                     gcagtCTGGATACTGCCCGCCGAGGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATATTCCGGCAAAGTGAATACAGAGCAGTGCCATGCCAATCAGCAGAGCCGCCGGCATGGATGTTGTCGc                                                                                                              <  2:518125/136‑1 (MQ=255)                       gTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCtt                                                                                                            <  2:337943/140‑1 (MQ=255)                         tCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTtgt                                                                                                          <  2:157494/140‑1 (MQ=255)                                 cTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTAt                                                                                                  <  1:133982/140‑1 (MQ=255)                                    cccACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAg                                                                                               >  1:168951/1‑140 (MQ=255)                                    cccACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAg                                                                                               >  1:191979/1‑140 (MQ=255)                                    cccACCGACGCAGTCATGCGAGAAGCAACCGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTactgt                                                                                               >  1:518125/1‑136 (MQ=255)                                            cGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAgg                                                                                       >  2:361559/1‑140 (MQ=255)                                                  cATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGcag                                                                                 <  1:254012/140‑1 (MQ=255)                                                           aGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAgaacagaaca                                                                        >  1:188387/1‑140 (MQ=255)                                                                    gACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCaa                                                               <  2:191979/140‑1 (MQ=255)                                                                          gATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGc                                                         >  2:247643/1‑140 (MQ=255)                                                                                  cccAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTa                                                 <  1:199707/140‑1 (MQ=255)                                                                                          gATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCtttt                                         >  1:356892/1‑140 (MQ=255)                                                                                          gATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCtttt                                         >  2:312433/1‑140 (MQ=255)                                                                                               tCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCg                                    <  1:312433/140‑1 (MQ=255)                                                                                                  ggCAAAGTGAATACAAAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCg                                    >  2:250034/1‑137 (MQ=255)                                                                                                  ggCAAAGTGAATACAAAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCg                                    <  1:250034/137‑1 (MQ=255)                                                                                                    cAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCGGAGcc                               >  2:465501/1‑140 (MQ=255)                                                                                                              aCAGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCGGAGCCATTCCCCCtt                     <  2:188387/140‑1 (MQ=255)                                                                                                                aGAACAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCGGAGCCATTCCCCCTTTg                   >  1:401447/1‑140 (MQ=255)                                                                                                                   aCAGTGCCATGCCAATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCGGAGCCATTCCCCCTTTGAcc                >  2:443542/1‑140 (MQ=255)                                                                                                                        gccatgccaATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCGGAGCCATTCCCCCTTTGAccagcca           <  1:443542/140‑1 (MQ=255)                                                                                                                         ccatgccaATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCGGAGCCATTCCCCCTTTGAccagccag          >  1:223883/1‑140 (MQ=255)                                                                                                                           atgccaATCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCGGAGCCATTCCCCCTTTGCCCAGCCAGTc        <  2:401447/140‑1 (MQ=255)                                                                                                                                 aTCAGCAGAGCCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCGGAGCCATTCCCCCTTTGACCAGCCAGTCGgtaac  >  2:145397/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   AGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGATCAAGCCCCAGCAGGATGTTCCGGCAAAGTGAATACAGAACAGTGCCATGCCAATCAGCAGAACCGCCGTCATGGATGTTGTCGCTTGTGGTACTATCAGCGTAAAGGCGGCAGAACAGAACATCCCGGCAATAATGCAGATCTTACGGCTTTTAATCGGAGCCATTCCCCCTTTGACCAGCCAGTCGGTAAC  >  NC_000913/4593510‑4593777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑