Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,599,309	G→A	L45L (CTG→TTG)	opgB ←	phosphoglycerol transferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,599,309	0	G	A	100.0%	133.9 / NA	39	L45L (CTG→TTG)	opgB	phosphoglycerol transferase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/15);  total (24/15)

CCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGG  >  NC_000913/4599174‑4599444                                                                                                                                        |                                                                                                                                        ccGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTg                                                                                                                                     <  1:257803/140‑1 (MQ=255)       tCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATAttt                                                                                                                               >  2:270500/1‑140 (MQ=255)            cccAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAAc                                                                                                                          <  1:360617/140‑1 (MQ=255)                      tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGc                                                                                                                >  2:278834/1‑140 (MQ=255)                           aTACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAg                                                                                                           <  1:270500/140‑1 (MQ=255)                            tACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGc                                                                                                          <  2:365119/140‑1 (MQ=255)                            tACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGc                                                                                                          <  2:210253/140‑1 (MQ=255)                                                   aCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAacca                                                                                   <  1:372109/140‑1 (MQ=255)                                                    cGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAaccac                                                                                  >  2:194211/1‑140 (MQ=255)                                                    cGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAaccac                                                                                  <  1:278834/140‑1 (MQ=255)                                                       cAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAg                                                                               <  1:194211/140‑1 (MQ=255)                                                          cACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAaccac                                                                                  >  2:82928/1‑134 (MQ=255)                                                          cACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAaccac                                                                                  <  1:82928/134‑1 (MQ=255)                                                               gTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACg                                                                       >  2:213766/1‑140 (MQ=255)                                                                tCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGt                                                                      <  1:476870/140‑1 (MQ=255)                                                                          ggTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTcc                                                            >  2:4108/1‑140 (MQ=255)                                                                                         aaCCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGAGAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGca                                             >  1:472154/1‑140 (MQ=255)                                                                                         aaCCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGca                                             >  1:275416/1‑140 (MQ=255)                                                                                          aCCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAAt                                                  <  1:534309/134‑1 (MQ=255)                                                                                          aCCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAAt                                                  >  2:534309/1‑134 (MQ=255)                                                                                           ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGcaca                                           >  1:116373/1‑140 (MQ=255)                                                                                           ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGcaca                                           >  1:294521/1‑140 (MQ=255)                                                                                           ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGcaca                                           >  1:239078/1‑140 (MQ=255)                                                                                           ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGcaca                                           >  2:348785/1‑140 (MQ=255)                                                                                           ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGca                                             >  1:325517/1‑138 (MQ=255)                                                                                           ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGca                                             <  2:325517/138‑1 (MQ=255)                                                                                              cgtcgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACagag                                        <  1:316434/140‑1 (MQ=255)                                                                                              cgtcgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACagag                                        >  2:47964/1‑140 (MQ=255)                                                                                              cgtcgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACagag                                        >  2:353506/1‑140 (MQ=255)                                                                                              cgtcgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACagag                                        >  2:316434/1‑140 (MQ=255)                                                                                                           aTCGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAggg                           >  2:116505/1‑140 (MQ=255)                                                                                                             cGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCg                         >  2:340461/1‑140 (MQ=255)                                                                                                             cGCCAGTAAAATAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCg                         >  2:264836/1‑140 (MQ=255)                                                                                                              gCCAGTAAAATAGTCGCTGACAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGa                        >  1:211477/1‑140 (MQ=255)                                                                                                                     aaaTAGTCGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAagagag                 <  1:264836/140‑1 (MQ=255)                                                                                                                            cGCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCt          >  2:445578/1‑140 (MQ=255)                                                                                                                             gCTGGCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTg         >  1:303699/1‑140 (MQ=255)                                                                                                                                ggCAAACAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACa      >  2:534758/1‑140 (MQ=255)                                                                                                                                    aaCAAGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACgg  <  1:213766/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGG  >  NC_000913/4599174‑4599444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑