Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	269,687	T→C	T166A (ACG→GCG)	perR ←	putative transcriptional regulator PerR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	269,687	0	T	C	100.0%	153.0 / NA	42	T166A (ACG→GCG)	perR	putative transcriptional regulator PerR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/19);  total (23/19)

TTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGTAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCATATTCTGTGCTGGCTGAAAGTCGCAGATCGATGCTTGGG  >  NC_000913/269551‑269823                                                                                                                                         |                                                                                                                                         ttGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGTGGAGAACACAGTGGCGCAAg                                                                                                                                       >  2:235624/1‑140 (MQ=255)        gCGTCATCTTATTCGCCTCAAACCATCCTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCa                                                                                                                                >  1:531076/1‑140 (MQ=255)         cGTCATCTTATTCGCCTCAAACCATCCTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCaa                                                                                                                               >  2:268/1‑140 (MQ=255)              tCTTATTCGCCTCAAACCATCCTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCa                                                                                                                          >  1:99535/1‑140 (MQ=255)                       ccTCAAACCATCCTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGTGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGAtc                                                                                                                 <  1:235624/140‑1 (MQ=255)                        cTCAAACCATCCTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGa                                                                                                                   <  1:222178/137‑1 (MQ=255)                        cTCAAACCATCCTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGa                                                                                                                   >  2:222178/1‑137 (MQ=255)                              ccATCCTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCAt                                                                                                          <  1:520628/140‑1 (MQ=255)                                  ccTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGc                                                                                                                       <  1:286520/123‑1 (MQ=255)                                  ccTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGc                                                                                                                       >  2:286520/1‑123 (MQ=255)                                  ccTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAggg                                                                                                      >  2:236729/1‑140 (MQ=255)                                  ccTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAggg                                                                                                      >  1:85678/1‑140 (MQ=255)                                     ttCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGa                                                                                                   >  2:164861/1‑140 (MQ=255)                                        cACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATgg                                                                                                >  2:372924/1‑140 (MQ=255)                                           tGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTTTCATAGGGTGATGGGCg                                                                                             <  1:478365/140‑1 (MQ=255)                                           tGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCg                                                                                             <  1:201533/140‑1 (MQ=255)                                               aCAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGt                                                                                         <  2:95153/140‑1 (MQ=255)                                                       acaTCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCAcc                                                                                    <  1:103562/137‑1 (MQ=255)                                                       acaTCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCAcc                                                                                    >  2:103562/1‑137 (MQ=255)                                                       acaTCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTa                                                                                 <  2:85678/140‑1 (MQ=255)                                                       acaTCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTa                                                                                 >  2:67272/1‑140 (MQ=255)                                                        caTCGCACTGAATCAGTGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGt                                                                                         >  2:330701/1‑131 (MQ=255)                                                        caTCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGt                                                                                         <  1:330701/131‑1 (MQ=255)                                                                     cAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGAt                                                                   <  1:236729/140‑1 (MQ=255)                                                                            aaCGCATAGAGATCCTCTGGTTTCTTTTGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAatc                                                            >  2:353527/1‑140 (MQ=255)                                                                               gCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAatcatc                                                         >  2:283107/1‑140 (MQ=255)                                                                                         ccTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCaaaa                                               <  2:99535/140‑1 (MQ=255)                                                                                         ccTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCaaaa                                               <  2:260837/140‑1 (MQ=255)                                                                                         ccTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCaaaa                                               <  1:372924/140‑1 (MQ=255)                                                                                          ctctGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTc                                                   <  1:53710/135‑1 (MQ=255)                                                                                          ctctGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTc                                                   >  2:53710/1‑135 (MQ=255)                                                                                           tctGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACg                                             >  1:396802/1‑140 (MQ=255)                                                                                                    tttAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCTTATTc                                    >  1:424809/1‑140 (MQ=255)                                                                                                         ccGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGc                                          <  2:206938/129‑1 (MQ=255)                                                                                                         ccGCTCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGc                                          >  1:206938/1‑129 (MQ=255)                                                                                                             tCAGCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCATATTCTGTgctggc                           >  2:43147/1‑140 (MQ=255)                                                                                                                gCCAGTTGGGGAGAACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCATATTCTGTGCTGGCTGa                        <  1:283107/140‑1 (MQ=255)                                                                                                                           agaACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCATATTCTGTGCTGGCTGAAAGTCGCAGAt             <  1:89282/140‑1 (MQ=255)                                                                                                                           agaACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCATATTCTGTGCTGGCTGAAAGTCGCAGAt             <  1:67272/140‑1 (MQ=255)                                                                                                                           agaACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCATATTCTGTGCTGGCTGAAAGTCGCAGAt             >  1:251316/1‑140 (MQ=255)                                                                                                                           agaACACAGTGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCATATTCTGTGCTGGCTGAAAGTCGCAGAt             >  1:166150/1‑140 (MQ=255)                                                                                                                                    tGGCGCAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCATATTCTGTGCTGGCTGAAAGTCGCAGATCGATGCTTg    >  1:403983/1‑140 (MQ=255)                                                                                                                                      gcgcAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCATATTCTGTGCTGGCTGAAAGTCGCAGATCGATGCTTggg  <  2:290051/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTCCACTGGTACAACTGCACATCGCACTGAATCAATGTCAACGCATAGAGATCCTCTGGTTTCTTTAGCCGCTCAGCCAGTTGGGGAGAACACAGTGGCGTAAGTTCTTCAACAGCAAGCGGGATCTTCTCATAGGGTGATGGGCGGGGTTCACCGTAGACTATATCGAGATCAAAATCATCCTGTTCAAAACGTGCATATTCTGTGCTGGCTGAAAGTCGCAGATCGATGCTTGGG  >  NC_000913/269551‑269823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑