Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,696	C→T	P303L (CCG→CTG)	yagH →	CP4‑6 prophage; putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,696	0	C	T	100.0%	66.5 / NA	20	P303L (CCG→CTG)	yagH	CP4‑6 prophage; putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/7);  total (13/7)

CATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCC  >  NC_000913/287568‑287827                                                                                                                                 |                                                                                                                                    cATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAg                                                                                                                          >  1:301039/1‑140 (MQ=255)      ccTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAggcggc                                                                                                                     >  1:47994/1‑140 (MQ=255)             cACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCAcg                                                                                                              <  2:47994/140‑1 (MQ=255)                cAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACgcgc                                                                                                           >  2:301486/1‑140 (MQ=255)                cAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACgcgc                                                                                                           >  1:94153/1‑140 (MQ=255)                    cgcccgcTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCt                                                                                                       >  1:270750/1‑140 (MQ=255)                                          tGCCGCTGCTGGCCTCCGGCAGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTa                                                                                 <  1:26523/140‑1 (MQ=255)                                                  ctgGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGagca                                                                         >  2:263334/1‑140 (MQ=255)                                                     gCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTTACCGTGAAAGGCCCGCAAGTAGCCGAGCAGcc                                                                      <  2:270750/140‑1 (MQ=255)                                                                            cccGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGc                                                      <  2:373421/133‑1 (MQ=255)                                                                            cccGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGc                                                      >  1:373421/1‑133 (MQ=255)                                                                                cTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGa                                           <  1:190275/140‑1 (MQ=255)                                                                                cTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGa                                           >  2:190275/1‑140 (MQ=255)                                                                                 tGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGAc                                          >  1:185353/1‑140 (MQ=255)                                                                                   gggCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGa                                        >  2:55760/1‑140 (MQ=255)                                                                                                              gAATGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGa             <  1:301486/140‑1 (MQ=255)                                                                                                                 tGGCGCGACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCt          >  1:313676/1‑140 (MQ=255)                                                                                                                    cgcgACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCa       >  2:238913/1‑140 (MQ=255)                                                                                                                      cgACGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGa     <  1:238913/140‑1 (MQ=255)                                                                                                                         cGGCTGGCTGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGAccc  >  1:274894/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                    CATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCC  >  NC_000913/287568‑287827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑