Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,988	C→G	A167G (GCC→GGC)	nhaA →	Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,988	0	C	G	100.0%	139.2 / NA	38	A167G (GCC→GGC)	nhaA	Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (22/16);  total (22/16)

GAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGG  >  NC_000913/17858‑18123                                                                                                                                   |                                                                                                                                        gAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTat                                                                                                                                >  1:83586/1‑140 (MQ=255)  aaGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTatc                                                                                                                               >  1:536954/1‑140 (MQ=255)   aGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTatca                                                                                                                              >  2:434813/1‑140 (MQ=255)   aGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTatca                                                                                                                              <  2:231854/140‑1 (MQ=255)        gggCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCa                                                                                                                         <  1:165241/140‑1 (MQ=255)            gATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGt                                                                                                                     <  2:193419/140‑1 (MQ=255)             aTCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGtt                                                                                                                    <  2:419873/140‑1 (MQ=255)               cccGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCa                                                                                                                         <  1:301344/133‑1 (MQ=255)               cccGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCa                                                                                                                         >  2:301344/1‑133 (MQ=255)               cccGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCt                                                                                                                  <  1:279499/140‑1 (MQ=255)               cccGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCt                                                                                                                  >  2:192520/1‑140 (MQ=255)                     ggcTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTa                                                                                                            <  2:1735/140‑1 (MQ=255)                     ggcTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTa                                                                                                            <  2:536954/140‑1 (MQ=255)                                 tGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGa                                                                                                >  1:445388/1‑140 (MQ=255)                                             tGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTg                                                                                    <  2:432319/140‑1 (MQ=255)                                             tGGTATACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTg                                                                                    >  2:142714/1‑140 (MQ=255)                                                          cTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGt                                                                       <  1:515262/140‑1 (MQ=255)                                                          cTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGt                                                                       >  2:161937/1‑140 (MQ=255)                                                                  aaGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTg                                                               <  2:438268/140‑1 (MQ=255)                                                                       gtgtTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTa                                                          >  2:297746/1‑140 (MQ=255)                                                                        tgtTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTAc                                                         <  2:261935/140‑1 (MQ=255)                                                                               ttAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGt                                                  >  2:50318/1‑140 (MQ=255)                                                                                        aaGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCt                                         >  1:339931/1‑140 (MQ=255)                                                                                             cTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTcg                                                      <  1:165129/122‑1 (MQ=255)                                                                                             cTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTcg                                                      >  2:165129/1‑122 (MQ=255)                                                                                                       gCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCACATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCa                               <  1:309759/135‑1 (MQ=255)                                                                                                       gCTCTGGCTATTATCGACGATCTTGGGGGCATCATTATCATCACATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCa                               >  2:309759/1‑135 (MQ=255)                                                                                                                attatCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGt                 <  2:445388/140‑1 (MQ=255)                                                                                                                 ttatCGACGATCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTc                >  2:25077/1‑140 (MQ=255)                                                                                                                     cgacgaTCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCtata            >  1:213635/1‑140 (MQ=255)                                                                                                                       acgaTCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATAtt          >  1:426457/1‑140 (MQ=255)                                                                                                                       acgaTCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATAtt          >  2:338080/1‑140 (MQ=255)                                                                                                                       acgaTCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATAtt          >  2:373840/1‑140 (MQ=255)                                                                                                                       acgaTCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATAtt          >  1:9914/1‑140 (MQ=255)                                                                                                                           tCTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCttg      >  2:49678/1‑140 (MQ=255)                                                                                                                            cTTGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCttgt     >  1:175502/1‑140 (MQ=255)                                                                                                                              tGGGGGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCttgttg   <  1:50318/140‑1 (MQ=255)                                                                                                                               ggggGCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTgg  >  1:137521/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        GAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGG  >  NC_000913/17858‑18123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑