Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	303,267	A→G	T93A (ACC→GCC)	yagU →	inner membrane protein that contributes to acid resistance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	303,267	0	A	G	100.0%	155.4 / NA	43	T93A (ACC→GCC)	yagU	inner membrane protein that contributes to acid resistance
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

TAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGT  >  NC_000913/303134‑303398                                                                                                                                      |                                                                                                                                    tAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTg                                                                                                                               >  2:527086/1‑140 (MQ=255)   aTGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGcc                                                                                                                             >  2:413815/1‑140 (MQ=255)   aTGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGcc                                                                                                                             >  2:123566/1‑140 (MQ=255)    tGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCg                                                                                                                            >  1:145503/1‑140 (MQ=255)    tGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCg                                                                                                                            >  1:300467/1‑140 (MQ=255)       aGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGc                                                                                                                         >  2:193194/1‑140 (MQ=255)       aGCGTGTGGCCCGAAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGc                                                                                                                         <  1:399479/140‑1 (MQ=255)        gCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCa                                                                                                                        <  1:527086/140‑1 (MQ=255)         cGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGc                                                                                                                         <  1:69062/138‑1 (MQ=255)         cGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGc                                                                                                                         >  2:69062/1‑138 (MQ=255)           tgtgGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGc                                                                                                                         >  2:29997/1‑136 (MQ=255)           tgtgGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGc                                                                                                                         <  1:29997/136‑1 (MQ=255)              ggCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCtt                                                                                                                  >  2:165335/1‑140 (MQ=255)                 ccGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTaa                                                                                                               <  1:53125/140‑1 (MQ=255)                  cGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAAc                                                                                                              <  1:88742/140‑1 (MQ=255)                                  ggCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAAc                                                                                                              <  1:485815/124‑1 (MQ=255)                                  ggCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAAc                                                                                                              >  2:485815/1‑124 (MQ=255)                                       cAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACAtt                                                                                         >  2:49252/1‑140 (MQ=255)                                            cAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCtt                                                                                    <  1:506219/140‑1 (MQ=255)                                                ttgattgCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCg                                                                                <  1:49252/140‑1 (MQ=255)                                                                            aCCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTgg                                                    <  1:65231/140‑1 (MQ=255)                                                                             ccGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGt                                                   <  1:460975/140‑1 (MQ=255)                                                                             ccGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGt                                                   <  1:72750/140‑1 (MQ=255)                                                                             ccGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGt                                                   <  1:410486/140‑1 (MQ=255)                                                                                        tcttcGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGGCTTTAACTGGGGTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTAt                                        >  1:281091/1‑140 (MQ=255)                                                                                          ttcGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTAttt                                      >  1:207175/1‑140 (MQ=255)                                                                                          ttcGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTAttt                                      >  2:283204/1‑140 (MQ=255)                                                                                            cGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTcc                                    <  1:191565/140‑1 (MQ=255)                                                                                            cGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTcc                                    <  2:207175/140‑1 (MQ=255)                                                                                                         ggCTGACAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGGTAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAActc                       >  1:240991/1‑140 (MQ=255)                                                                                                              aCAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCa                  >  1:505550/1‑140 (MQ=255)                                                                                                              aCAGATCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCa                  >  2:334103/1‑140 (MQ=255)                                                                                                                   tCCCAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCt             >  1:540734/1‑140 (MQ=255)                                                                                                                     ccAATGCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTa           <  2:145503/140‑1 (MQ=255)                                                                                                                          gCGGCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGc      <  2:300467/140‑1 (MQ=255)                                                                                                                            ggCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCa                  <  2:84007/126‑1 (MQ=255)                                                                                                                            ggCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCa                  >  1:84007/1‑126 (MQ=255)                                                                                                                            ggCTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAg    <  1:165335/140‑1 (MQ=255)                                                                                                                              cTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGc                   <  2:106207/123‑1 (MQ=255)                                                                                                                              cTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGc                   >  1:106207/1‑123 (MQ=255)                                                                                                                              cTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAg                 <  2:489438/125‑1 (MQ=255)                                                                                                                              cTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAg                 >  1:489438/1‑125 (MQ=255)                                                                                                                              cTGTTTATGCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGt  >  1:92269/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    TAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGACTGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGT  >  NC_000913/303134‑303398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑