Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	354,626	C→T	intergenic (+34/‑296)	prpE → / → codB	propionyl‑CoA synthetase/cytosine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,626	0	C	T	100.0%	90.1 / NA	27	intergenic (+34/‑296)	prpE/codB	propionyl‑CoA synthetase/cytosine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

TTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGC  >  NC_000913/354508‑354742                                                                                                                       |                                                                                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATg                                                                                                 >  2:147643/1‑140 (MQ=255) ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATg                                                                                                 >  1:480246/1‑140 (MQ=255)           cgcgATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                                       <  1:24610/140‑1 (MQ=255)                          cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGAcgc                                                                                     >  2:448697/1‑127 (MQ=255)                          cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGAcgc                                                                                     <  1:448697/127‑1 (MQ=255)                          cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                                       >  1:108918/1‑125 (MQ=255)                          cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                                       <  2:462078/125‑1 (MQ=255)                          cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                                       >  1:462078/1‑125 (MQ=255)                          cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                                       <  2:108918/125‑1 (MQ=255)                          cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                                        >  1:306370/1‑140 (MQ=255)                          cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                                        >  2:198772/1‑140 (MQ=255)                             acgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTAc                                                                     >  2:372377/1‑140 (MQ=255)                              cgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACg                                                                    <  2:264252/140‑1 (MQ=255)                                    ttGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTg                                                              >  1:520386/1‑140 (MQ=255)                                     tgatgaTCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGa                                                             >  1:450507/1‑140 (MQ=255)                                               gCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTc                                                   >  2:9547/1‑140 (MQ=255)                                                 gtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCgg                                                 >  1:165842/1‑140 (MQ=255)                                                                ccGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGt                                  <  1:9547/140‑1 (MQ=255)                                                                 cGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                                 <  1:372377/140‑1 (MQ=255)                                                                 cGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                                 <  1:333918/140‑1 (MQ=255)                                                                 cGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                                 >  2:333918/1‑140 (MQ=255)                                                                 cGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                                 <  2:450507/140‑1 (MQ=255)                                                                  gCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCg                                <  1:13425/140‑1 (MQ=255)                                                                        cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCa                              >  2:270408/1‑136 (MQ=255)                                                                        cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCa                              <  1:270408/136‑1 (MQ=255)                                                                                     ggTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAg             >  1:467666/1‑140 (MQ=255)                                                                                                gATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGc  >  1:139436/1‑140 (MQ=255)                                                                                                                       |                                                                                                                     TTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGC  >  NC_000913/354508‑354742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑