Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	366,116	A→G	F64L (TTT→CTT)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,116	0	A	G	100.0%	127.4 / NA	36	F64L (TTT→CTT)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/18);  total (18/18)

GGATAGGTCACGTTGGTGTAGATGGGCGCATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTT  >  NC_000913/365986‑366252                                                                                                                                   |                                                                                                                                         ggATAGGTCACGTTGGTGTAGATGGGCGCATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCaaa                                                                                                                                 >  1:95883/1‑140 (MQ=255)       gTCACGTTGGTGTAGATGGGCGCATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGcgcca                                                                                                                           >  1:13035/1‑140 (MQ=255)       gTCACGTTGGTGTAGATGGGCGCATCGTAACCGTGCATCTACCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGcgcca                                                                                                                           <  1:177555/140‑1 (MQ=255)            gTTGGTGTAGATGGGCGCATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGcgccattcgc                                                                                                                      >  2:169575/1‑140 (MQ=255)                         ggCGCATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTgcg                                                                                                         <  1:169575/140‑1 (MQ=255)                          gcgcATCGTAACCGTGCATCTGGCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTgcgc                                                                                                        <  1:216605/140‑1 (MQ=255)                          gcgcATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTgcgc                                                                                                        <  2:462453/140‑1 (MQ=255)                          gcgcATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTgcgc                                                                                                        <  2:455596/140‑1 (MQ=255)                           cgcATCGTAACCGTGCATCTGCCAGTTTGAGGTGACGACGACAGTATCGGCCTCAGGAAGATCGCTCTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTgcgc                                                                                                        <  1:215473/139‑1 (MQ=255)                           cgcATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGcgccattc                                                                                                                        >  2:323869/1‑123 (MQ=255)                           cgcATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGcgccattc                                                                                                                        <  1:323869/123‑1 (MQ=255)                           cgcATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTgcgc                                                                                                        >  2:215473/1‑139 (MQ=255)                           cgcATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCa                                                                                                       >  1:392740/1‑140 (MQ=255)                            gcATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCaa                                                                                                      <  2:392740/140‑1 (MQ=255)                             cATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAAc                                                                                                     >  2:427465/1‑140 (MQ=255)                               tCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTg                                                                                                   >  2:404716/1‑140 (MQ=255)                                cGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGt                                                                                                  >  2:212180/1‑140 (MQ=255)                                 gTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGtt                                                                                                 <  1:212180/140‑1 (MQ=255)                                        tGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAgg                                                                                          >  1:235294/1‑140 (MQ=255)                                             cTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGAt                                                                                     <  2:235294/140‑1 (MQ=255)                                                    tttGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCg                                                                              <  1:427465/140‑1 (MQ=255)                                                             acgacgacAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTc                                                                     <  1:38414/140‑1 (MQ=255)                                                               gacgacAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGc                                                                   >  2:235907/1‑140 (MQ=255)                                                                       tATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGc                                                           >  2:532216/1‑140 (MQ=255)                                                                          cGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAg                                                        <  1:404716/140‑1 (MQ=255)                                                                                       aTCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTgg                                                    >  2:259870/1‑131 (MQ=255)                                                                                       aTCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTgg                                                    <  1:259870/131‑1 (MQ=255)                                                                                       aTCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAgggg                                           >  1:152337/1‑140 (MQ=255)                                                                                        tCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAggggg                                          <  2:95883/140‑1 (MQ=255)                                                                                        tCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAggggg                                          <  2:152337/140‑1 (MQ=255)                                                                                           cACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAtg                                       <  1:532216/140‑1 (MQ=255)                                                                                                cagccagcTTTCCGGCACCGCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGtgctg                                  >  1:286938/1‑140 (MQ=255)                                                                                                                   gCTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGt               <  2:286938/140‑1 (MQ=255)                                                                                                                    cTTCTGGTGCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTa              >  2:199578/1‑140 (MQ=255)                                                                                                                            gCCGGAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAgg      >  2:415847/1‑140 (MQ=255)                                                                                                                                gAAGCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGttt  >  1:381928/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                         GGATAGGTCACGTTGGTGTAGATGGGCGCATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTT  >  NC_000913/365986‑366252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑