Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	467,420	T→C	R3R (CGT→CGC)	cof →	HMP‑PP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	467,420	0	T	C	100.0%	84.6 / NA	24	R3R (CGT→CGC)	cof	HMP‑PP phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/7);  total (17/7)

ACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAG  >  NC_000913/467285‑467555                                                                                                                                        |                                                                                                                                        aCTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTgg                                                                                                                                     <  2:42483/140‑1 (MQ=255)         aGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCAttt                                                                                                                             >  2:37374/1‑140 (MQ=255)               ttGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATatg                                                                                                                       >  1:7040/1‑140 (MQ=255)                 gAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATatgga                                                                                                                     <  2:7040/140‑1 (MQ=255)                 gAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATatgga                                                                                                                     <  1:37374/140‑1 (MQ=255)                                 aCCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATg                                                                                                     >  2:95110/1‑140 (MQ=255)                                     ggAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCg                                                                                                 >  2:265583/1‑140 (MQ=255)                                     ggAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGAGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCg                                                                                                 >  2:299888/1‑140 (MQ=255)                                                 tttAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAg                                                                                     <  1:40732/140‑1 (MQ=255)                                                    aaCTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTg                                                                                  >  2:341452/1‑140 (MQ=255)                                                        aTTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGaa                                                                              >  2:265499/1‑140 (MQ=255)                                                              tATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCct                                                                        <  1:95110/140‑1 (MQ=255)                                                                      ccGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACtt                                                                >  1:290171/1‑140 (MQ=255)                                                                        gTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTg                                                              >  1:68440/1‑140 (MQ=255)                                                                        gTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTg                                                              >  2:59168/1‑140 (MQ=255)                                                                                         cTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAAcg                                             <  1:341452/140‑1 (MQ=255)                                                                                                aCCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACAtt                                      >  2:292644/1‑140 (MQ=255)                                                                                                            gtCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACtttt                          >  1:174872/1‑140 (MQ=255)                                                                                                            gtCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACtttt                          >  1:410764/1‑140 (MQ=255)                                                                                                            gtCACAGAGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACtttt                          >  1:159973/1‑140 (MQ=255)                                                                                                                       gagaAAGAAATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCg               >  2:164647/1‑140 (MQ=255)                                                                                                                        agaaagaaATGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGt              >  1:58609/1‑140 (MQ=255)                                                                                                                                aTGGCTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCt      <  1:275702/140‑1 (MQ=255)                                                                                                                                    cTCGCCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGgag  >  2:397967/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAG  >  NC_000913/467285‑467555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑