Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,375	A→G	T329A (ACC→GCC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,375	0	A	G	100.0%	78.8 / NA	23	T329A (ACC→GCC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/11);  total (12/11)

GTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCG  >  NC_000913/23241‑23510                                                                                                                                       |                                                                                                                                        gtctCGGCGTGACCGATTACCCCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTGCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATgccggtgccggt                                                                                                                                    <  2:129383/137‑1 (MQ=255)  tATCGGCGTGACCGATTACACGATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGccggtgccggtg                                                                                                                                   <  2:358917/140‑1 (MQ=255)     cGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGgtgccggtgccg                                                                                                                                >  1:203872/1‑140 (MQ=255)           gACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTcaca                                                                                                                          <  1:512671/140‑1 (MQ=255)                         ttCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCAc                                                                                                            >  2:439963/1‑140 (MQ=255)                          tctcGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACg                                                                                                           >  2:205605/1‑140 (MQ=255)                          tctcGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACg                                                                                                           <  2:203872/140‑1 (MQ=255)                              ggCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGccc                                                                                                       >  1:313282/1‑140 (MQ=255)                              ggCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGccc                                                                                                       <  2:313282/140‑1 (MQ=255)                                   ggTAAAAGGTTCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacg                                                                                                  >  2:159066/1‑140 (MQ=255)                                        aaGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAt                                                                                             >  1:276220/1‑140 (MQ=255)                                        aaGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAt                                                                                             >  2:263434/1‑140 (MQ=255)                                            tGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGa                                                                                         <  1:91428/140‑1 (MQ=255)                                            tGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGa                                                                                         <  1:263434/140‑1 (MQ=255)                                             gCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGAt                                                                                        <  1:530760/140‑1 (MQ=255)                                                           gctgcGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACg                                                                          >  1:46527/1‑140 (MQ=255)                                                                         aTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATAc                                                                           <  2:59435/125‑1 (MQ=255)                                                                         aTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATAc                                                                           >  1:59435/1‑125 (MQ=255)                                                                                     gCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGc                                                >  1:7952/1‑140 (MQ=255)                                                                                         cGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGAGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCgg                                            >  1:252718/1‑140 (MQ=255)                                                                                          gACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGa                                           <  1:535509/140‑1 (MQ=255)                                                                                          gACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGa                                           <  2:46527/140‑1 (MQ=255)                                                                                                             gCGATCACGTTACCCTGGATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGc                        <  1:284680/140‑1 (MQ=255)                                                                                                                             ggATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCggg                         >  1:387595/1‑123 (MQ=255)                                                                                                                             ggATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCggg                         <  2:387595/123‑1 (MQ=255)                                                                                                                             ggATGCCGGTGCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGTACTTATCCGACGCTgg        >  2:224707/1‑140 (MQ=255)                                                                                                                                   cggtgccggtgcCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg  >  2:430950/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCG  >  NC_000913/23241‑23510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑