Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	575,712	A→G	S14S (AGT→AGC)	insH‑2 ←	DLP12 prophage; IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	575,712	0	A	G	100.0%	20.0 / NA	8	S14S (AGT→AGC)	insH‑2	DLP12 prophage; IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/5);  total (3/5)

CTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATAT  >  NC_000913/575654‑575843                                                           |                                                                                                                                    ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGGCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGaa                                                    <  1:207427/140‑1 (MQ=17)  ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGGCTCATGATGAACCCCGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAg                                                   <  2:57478/140‑1 (MQ=18)              aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGGCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCa                                       >  1:53986/1‑140 (MQ=255)              aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGGCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCa                                       <  2:349300/140‑1 (MQ=255)                        ctgctgAATTCACTGTCGGCGAAGGTAAGTTGATGGCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAg                                     <  1:417503/132‑1 (MQ=255)                        ctgctgAATTCACTGTCGGCGAAGGTAAGTTGATGGCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAg                                     >  2:417503/1‑132 (MQ=255)                               aTTCACTGTCGGCGAAGGTAAGTTGATGGCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTa                      <  1:300017/140‑1 (MQ=255)                                                   aGTTGATGGCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACatat  >  2:276910/1‑140 (MQ=255)                                                           |                                                                                                                                    CTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATAT  >  NC_000913/575654‑575843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑