Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	618,449	A→G	T66A (ACC→GCC)	fepE →	polysaccharide co‑polymerase family protein FepE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	618,449	0	A	G	100.0%	67.4 / NA	20	T66A (ACC→GCC)	fepE	polysaccharide co‑polymerase family protein FepE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/16);  total (4/16)

CCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGACCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTACAGAAGCATTTAACCTGTTTATCAA  >  NC_000913/618318‑618579                                                                                                                                    |                                                                                                                                   ccAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGcgcg                                                                                                                            <  1:237318/140‑1 (MQ=255)                                   ctcAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGc                                                                                          <  2:249589/140‑1 (MQ=255)                                                          aaaCGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACt                                                                   <  1:149597/140‑1 (MQ=255)                                                               gTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCt                                                              <  2:468551/140‑1 (MQ=255)                                                                 cATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTc                                                            <  1:213486/140‑1 (MQ=255)                                                                       ggTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGc                                                      <  1:341306/140‑1 (MQ=255)                                                                            tttttGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGctggat                                                 <  1:309363/140‑1 (MQ=255)                                                                                      tGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCCCGCCTCCAGAAACTGTTCAGTCGCCAGAGCTGGAGAAATCAATCACTAAGGTTCCGGTGCTGGATCTGGATATCa                                       >  2:248504/1‑140 (MQ=255)                                                                                           gcgcAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAAtt                                  <  1:273470/140‑1 (MQ=255)                                                                                                ggCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCg                             <  2:325058/140‑1 (MQ=255)                                                                                                  cTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTa                           <  1:190195/140‑1 (MQ=255)                                                                                                               ttCATCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTACAGAAGCATTTaa              <  1:121386/140‑1 (MQ=255)                                                                                                                  aTCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTACAGAAGCATTTAACCt           <  1:59990/140‑1 (MQ=255)                                                                                                                   tCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTACAGAAGCATTTAAc             >  1:391506/1‑137 (MQ=255)                                                                                                                   tCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTACAGAAGCATTTAAc             <  2:391506/137‑1 (MQ=255)                                                                                                                   tCCTGCCGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTACAGAAGCATTTAACCTg          >  2:314075/1‑140 (MQ=255)                                                                                                                        ccGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTACAGAAGCATTTAACCTGTTTAt     <  1:462656/140‑1 (MQ=255)                                                                                                                        ccGCAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAAAACCTGCTCAGTGGCAAGAGGTGGAGAAAACATTCACTAAGCTTCGTGTGCTGGATTTGGATATCAAAATTGATCGGACCGAAGCATTTAACCTtattat     >  2:231992/1‑140 (MQ=255)                                                                                                                           cAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTACAGAAGCATTTAACCTGTTTATCaa  <  1:314075/140‑1 (MQ=255)                                                                                                                           cAAAAATGGGCCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTACAGAAGCATTTAACCTGTTTATCaa  <  1:516885/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   CCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGGACCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAAGAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGTACAGAAGCATTTAACCTGTTTATCAA  >  NC_000913/618318‑618579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑