Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	621,770	A→G	S140S (AGT→AGC)	fepD ←	ferric enterobactin ABC transporter membrane subunit FebD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	621,770	0	A	G	100.0%	51.0 / NA	15	S140S (AGT→AGC)	fepD	ferric enterobactin ABC transporter membrane subunit FebD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/8);  total (7/8)

GCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGC  >  NC_000913/621640‑621889                                                                                                                                   |                                                                                                                        gCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCTATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTa                                                                                                                <  2:501191/140‑1 (MQ=255)      aaaCGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGc                                                                                                           <  1:424157/140‑1 (MQ=255)          gCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGcaaca                                                                                                       <  1:99162/140‑1 (MQ=255)                    gTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAgg                                                                                             >  2:442489/1‑140 (MQ=255)                     tAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGc                                                                                            <  1:286428/140‑1 (MQ=255)                               gATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGcc                                                                                  <  1:68283/140‑1 (MQ=255)                                  tAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGTCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCg                                                                               <  2:271579/140‑1 (MQ=255)                                                           tCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCt                                                      >  1:249490/1‑140 (MQ=255)                                                                         ccgccAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTaa                                        >  1:49259/1‑140 (MQ=255)                                                                                     cgcccgccAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGcgcc                            <  2:112032/140‑1 (MQ=255)                                                                                                    aaCGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGcgccg                           >  1:26347/1‑126 (MQ=255)                                                                                                    aaCGCACCGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGcgccg                           <  2:26347/126‑1 (MQ=255)                                                                                                          ccGGACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAg       >  1:171376/1‑140 (MQ=255)                                                                                                             gACTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTg    >  2:2383/1‑140 (MQ=255)                                                                                                               cTTAACTGCCCGCCGCCCTGGCTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGc  >  1:112479/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                        GCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGC  >  NC_000913/621640‑621889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑