Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	634,844	C→T	G178E (GGA→GAA)	ybdM ←	ParB‑like nuclease domain‑containing protein YbdM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	634,844	0	C	T	100.0%	67.8 / NA	21	G178E (GGA→GAA)	ybdM	ParB‑like nuclease domain‑containing protein YbdM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/9);  total (12/9)

GCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGT  >  NC_000913/634717‑634980                                                                                                                                |                                                                                                                                         gcagcaGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGc                                                                                                                              <  1:234184/140‑1 (MQ=255)        cTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAAtttt                                                                                                                       >  1:321691/1‑140 (MQ=255)             cGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTtattat                                                                                                                  <  2:321691/140‑1 (MQ=255)                     ccAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGtcccat                                                                                                          >  1:432511/1‑140 (MQ=255)                      cAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGtcccatc                                                                                                         <  1:361843/140‑1 (MQ=255)                                  tttAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTaa                                                                                             >  1:426645/1‑140 (MQ=255)                                   ttAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAg                                                                                            <  2:432511/140‑1 (MQ=255)                                   ttAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAg                                                                                            >  1:6940/1‑140 (MQ=255)                                                    ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCg                                                                           >  1:140482/1‑140 (MQ=255)                                                    ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCg                                                                           >  2:89060/1‑140 (MQ=255)                                                    ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCg                                                                           >  2:288843/1‑140 (MQ=255)                                                       atatTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACa                                                                        <  2:459419/140‑1 (MQ=255)                                                            gACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCg                                                                   <  1:454874/140‑1 (MQ=255)                                                                gTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGa                                                               >  2:160870/1‑140 (MQ=255)                                                                     cAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGa                                                          <  1:288843/140‑1 (MQ=255)                                                                                                   tCAGGCGCAGCACTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTTATTTTATGGGGTCCACGATCGCGATTATGACGAATa                            >  2:227156/1‑140 (MQ=255)                                                                                                           agcaCTTCGTCGCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCgcggc                    <  1:527076/140‑1 (MQ=255)                                                                                                                 tcgtcgCTGTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGTTGGGGTCCACGAGCGCGATTATGATGAATAGTGGCGGCCATTCg              >  1:390313/1‑140 (MQ=255)                                                                                                                         gTCCATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTAc      >  1:119257/1‑140 (MQ=255)                                                                                                                            cATTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTAcgcg   >  1:30633/1‑140 (MQ=255)                                                                                                                             aTTTCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt  <  2:48998/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                         GCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGT  >  NC_000913/634717‑634980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑