Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	659,197	C→A	intergenic (+47/+54)	tatE → / ← lipA	twin arginine protein translocation system ‑ TatE protein/lipoyl synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	659,197	0	C	A	100.0%	133.4 / NA	39	intergenic (+47/+54)	tatE/lipA	twin arginine protein translocation system ‑ TatE protein/lipoyl synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/19);  total (20/19)

GTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGT  >  NC_000913/659060‑659328                                                                                                                                          |                                                                                                                                    gTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAacc                                                                                                                                   <  2:40502/140‑1 (MQ=255)   tCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGc                                                                                                                                 >  1:501994/1‑140 (MQ=255)    cAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCt                                                                                                                                >  1:132532/1‑140 (MQ=255)          ggCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCaaaa                                                                                                                          >  1:47872/1‑140 (MQ=255)            cGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAg                                                                                                                        >  1:366699/1‑140 (MQ=255)            cGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAg                                                                                                                        <  2:110831/140‑1 (MQ=255)                  atgatgACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGGTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGtt                                                                                                                  <  2:253370/140‑1 (MQ=255)                  atgatgACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGtt                                                                                                                  <  2:352760/140‑1 (MQ=255)                       gaCGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTa                                                                                                             <  2:366699/140‑1 (MQ=255)                        aCGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTAt                                                                                                            <  2:132532/140‑1 (MQ=255)                           aTGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATcag                                                                                                         >  1:201964/1‑140 (MQ=255)                           aTGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATcag                                                                                                         >  2:378323/1‑140 (MQ=255)                            tgctgcGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAACAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATcaga                                                                                                        <  2:501994/140‑1 (MQ=255)                             gctgcGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATcagac                                                                                                       <  1:455687/140‑1 (MQ=255)                               tgcGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATcagacag                                                                                                     >  2:339206/1‑140 (MQ=255)                                  ggcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATg                                                                                                  <  1:45014/140‑1 (MQ=255)                                     gAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaa                                                                                               <  1:508749/140‑1 (MQ=255)                                     gAAAAAAGGCACCGACGTTGATCTTCAAACTGAAAAGCTCTCACATAAAGCGTGACGTGGTGAGCTGGACCAGCGCTAAATAACATGTACGGTACAAAAAAACCGCATCAAAAAGCGGGTTTTTTATCAGACAGGTgtaa                                                                                               <  2:504110/140‑1 (MQ=255)                                          aaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAat                                                                                          <  2:201964/140‑1 (MQ=255)                                                      ttGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGAtt                                                                              >  1:147280/1‑140 (MQ=255)                                                      ttGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGAtt                                                                              >  1:401801/1‑140 (MQ=255)                                                          tCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACtt                                                                          <  2:371151/140‑1 (MQ=255)                                                                                    aaGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAAt                                                >  2:238684/1‑140 (MQ=255)                                                                                         tGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                                           >  2:201639/1‑140 (MQ=255)                                                                                          gACGTGGCGAGCAGGACGCTCCCTCATTATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTACAAATCGGCGt                                          <  1:378323/140‑1 (MQ=255)                                                                                           aCGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCg                                              >  2:327614/1‑135 (MQ=255)                                                                                           aCGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCg                                              <  1:327614/135‑1 (MQ=255)                                                                                           aCGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTg                                         >  2:279989/1‑140 (MQ=255)                                                                                               ggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaa                                     <  1:339206/140‑1 (MQ=255)                                                                                                  gAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaagaa                                  >  1:320877/1‑140 (MQ=255)                                                                                                      aGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGc                                            >  1:501619/1‑126 (MQ=255)                                                                                                      aGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGc                                            <  2:124628/126‑1 (MQ=255)                                                                                                      aGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGc                                            <  2:501619/126‑1 (MQ=255)                                                                                                      aGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGc                                            >  1:124628/1‑126 (MQ=255)                                                                                                       ggACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCg                             <  2:47872/140‑1 (MQ=255)                                                                                                              cccTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCATTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAAc                      >  2:506532/1‑140 (MQ=255)                                                                                                                  cAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGAc                  >  1:483139/1‑140 (MQ=255)                                                                                                                  cAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGAc                  >  1:25611/1‑140 (MQ=255)                                                                                                                        cTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATg            <  1:238684/140‑1 (MQ=255)                                                                                                                         ttGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATgc           <  1:337465/140‑1 (MQ=255)                                                                                                                           gTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATgcag         >  1:524245/1‑140 (MQ=255)                                                                                                                                 tACAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATggg   >  2:323707/1‑140 (MQ=255)                                                                                                                                  aCAAAAAAACCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGt  <  1:174283/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    GTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGT  >  NC_000913/659060‑659328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑