Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	28,668	A→G	M99V (ATG→GTG)	dapB →	4‑hydroxy‑tetrahydrodipicolinate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	28,668	0	A	G	100.0%	90.1 / NA	26	M99V (ATG→GTG)	dapB	4‑hydroxy‑tetrahydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

GCCGGGAAAACAGGCGTTACCGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGATGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCTTTGCTGCCAATTTTAGCGTTGGCGTTAACGTCATGCTTAAGCTGCTGGAGAA  >  NC_000913/28536‑28798                                                                                                                                     |                                                                                                                                   gCCGGGAAAACAGGCGTTACCGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGAt                                                                                                                             <  2:279389/140‑1 (MQ=255)    gggAAAACAGGCGTTACCGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCgg                                                                                                                          <  1:237797/140‑1 (MQ=255)     ggAAAACAGGCGTTACCGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGc                                                                                                                         >  2:45439/1‑140 (MQ=255)     ggAAAACAGGCGTTACCGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGc                                                                                                                         >  1:62237/1‑140 (MQ=255)           cAGGCGTTACCGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACg                                                                                                                   <  1:45439/140‑1 (MQ=255)                   aCCGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGa                                                                                                           >  1:443506/1‑140 (MQ=255)                     cGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACg                                                                                                         <  1:374114/140‑1 (MQ=255)                                           gTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCg                                                                                   >  1:118504/1‑140 (MQ=255)                                           gTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCg                                                                                   >  2:244197/1‑140 (MQ=255)                                                aGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGAcg                                                                              <  2:443506/140‑1 (MQ=255)                                                 gatgatTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGAcgc                                                                             <  2:531706/140‑1 (MQ=255)                                                  atgatTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGAcgcc                                                                            <  1:78483/140‑1 (MQ=255)                                                           aTGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGat                                                                   >  1:224800/1‑140 (MQ=255)                                                                tttATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGc                                                              >  1:429451/1‑140 (MQ=255)                                                                     cGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTg                                                         >  1:184973/1‑140 (MQ=255)                                                                          ttACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGatat                                                                 >  1:478263/1‑127 (MQ=255)                                                                          ttACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGatat                                                                 <  2:478263/127‑1 (MQ=255)                                                                          ttACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCttt                                                    >  2:380870/1‑140 (MQ=255)                                                                          ttACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCttt                                                    >  1:213281/1‑140 (MQ=255)                                                                               cGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCTTtgctgc                                               <  2:213281/140‑1 (MQ=255)                                                                               cGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCTTtgctgc                                               <  1:244197/140‑1 (MQ=255)                                                                                                  aCCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCTTTGCTGCCAATTTTAGCGt                                   <  2:31442/133‑1 (MQ=255)                                                                                                  aCCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCTTTGCTGCCAATTTTAGCGt                                   >  1:31442/1‑133 (MQ=255)                                                                                                  aCCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCTTTGCTGCCAATTTTAGCGTTGGCGtt                            <  2:501553/140‑1 (MQ=255)                                                                                                               ttGTCGCCAGCATGGCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCTTTGCTGCCAATTTTAGCGTTGGCGTTAACGTCATGCTTa               <  1:380870/140‑1 (MQ=255)                                                                                                                            ggCAAAGGGGTGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCTTTGCTGCCAATTTTAGCGTTGGCGTTAACGTCATGCTTAAGCTGCTGGAGaa  <  2:62237/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   GCCGGGAAAACAGGCGTTACCGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGATGGTGATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGATATTGCGATTGTCTTTGCTGCCAATTTTAGCGTTGGCGTTAACGTCATGCTTAAGCTGCTGGAGAA  >  NC_000913/28536‑28798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑