Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	949,458	A→G	S37S (AGT→AGC)	ycaN ←	putative LysR‑type transcriptional regulator YcaN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	949,458	0	A	G	100.0%	107.6 / NA	30	S37S (AGT→AGC)	ycaN	putative LysR‑type transcriptional regulator YcaN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/16);  total (14/16)

TGATCTGAATTTCGTCGAAAGCTGGGCGCAATCGTTCATAGAGATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTA  >  NC_000913/949331‑949587                                                                                                                                |                                                                                                                                  tGATCTGAATTTCGTCGAAAGCTGGGCGCAATCGTTCATAGAGATTAGAACCTGCTTCCGTTAACGATACGCTTCGGGTTGTCAGATTGAAAAAGCGAATTTTAAGTCGTTGTTCCAGTGTTTTAATGATATGGCTAATg                                                                                                                       <  2:7402/140‑1 (MQ=255)   aTCTGAATTTCGTCGAAAGCTGGGCGCAATCGTTCATAGAGATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTTAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGc                                                                                                                     >  2:471666/1‑140 (MQ=255)   aTCTGAATTTCGTCGAAAGCTGGGCGCAATCGTTCATAGAGATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGc                                                                                                                     >  1:370227/1‑140 (MQ=255)   aTCTGAATTTCGTCGAAAGCTGGGCGCAATCGTTCATAGAGATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGc                                                                                                                     >  1:132397/1‑140 (MQ=255)          tttCGTCGAAAGCTGGGCGCAATCGTTCATAGAGATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATgg                                                                                                                      >  2:80634/1‑132 (MQ=255)          tttCGTCGAAAGCTGGGCGCAATCGTTCATAGAGATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATgg                                                                                                                      <  1:80634/132‑1 (MQ=255)             cgtcgAAAGCTGGGCGCAATCGTTCATAGAGATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGAt                                                                                                           >  2:491328/1‑140 (MQ=255)                   aaGCTGGGCGCAATCGTTCATAGAGATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCt                                                                                                     <  2:386929/140‑1 (MQ=255)                                tCGTTCATAGAGATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTTAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCg                                                                                        <  1:471666/140‑1 (MQ=255)                                        agagATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGa                                                                                <  2:491163/140‑1 (MQ=255)                                          agaTTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGaaa                                                                              <  2:137486/140‑1 (MQ=255)                                            aTTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGc                                                                            >  2:175598/1‑140 (MQ=255)                                            aTTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGc                                                                            >  2:72021/1‑140 (MQ=255)                                              tAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAGGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCtt                                                                          >  2:462753/1‑140 (MQ=255)                                                 aaCCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGa                                                                       >  2:96010/1‑140 (MQ=255)                                                      gCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACg                                                                  >  1:534730/1‑140 (MQ=255)                                                                  gATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAGGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCaaa                                                      <  1:462753/140‑1 (MQ=255)                                                                       gCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGc                                                               >  1:44867/1‑126 (MQ=255)                                                                       gCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGc                                                               <  2:44867/126‑1 (MQ=255)                                                                           cGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGc                                             <  1:491328/140‑1 (MQ=255)                                                                                tGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGt                                        <  2:132397/140‑1 (MQ=255)                                                                                tGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGt                                        <  1:96010/140‑1 (MQ=255)                                                                                  tCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCa                                      >  2:515276/1‑140 (MQ=255)                                                                                              aGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATc                          <  1:175598/140‑1 (MQ=255)                                                                                                  aaTTTTAAGACGTTGTTCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCa                      <  1:324600/140‑1 (MQ=255)                                                                                                               tgttCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATcc         <  2:312395/140‑1 (MQ=255)                                                                                                               tgttCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATcc         <  1:72021/140‑1 (MQ=255)                                                                                                               tgttCCAGTGTTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATcc         <  1:515276/140‑1 (MQ=255)                                                                                                                      gtgtTTTAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTa  >  1:208190/1‑140 (MQ=255)                                                                                                                           ttAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATc          <  1:530102/127‑1 (MQ=255)                                                                                                                           ttAATGCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATc          >  2:530102/1‑127 (MQ=255)                                                                                                                                |                                                                                                                                  TGATCTGAATTTCGTCGAAAGCTGGGCGCAATCGTTCATAGAGATTAGAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTA  >  NC_000913/949331‑949587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑