Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	951,066	A→G	G5G (GGT→GGC)	pflA ←	pyruvate formate‑lyase activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	951,066	0	A	G	100.0%	88.9 / NA	25	G5G (GGT→GGC)	pflA	pyruvate formate‑lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/14);  total (11/14)

TTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAA  >  NC_000913/950935‑951170                                                                                                                                    |                                                                                                         ttACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAAc                                                                                                  <  1:128755/140‑1 (MQ=255)   aCCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTg                                                                                                <  1:12570/140‑1 (MQ=255)       ccATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATa                                                                                                    >  1:164875/1‑132 (MQ=255)       ccATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATa                                                                                                    <  2:164875/132‑1 (MQ=255)              tgtCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGgtg                                                                                     <  1:445762/140‑1 (MQ=255)               gtCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGgtgt                                                                                    >  2:261993/1‑140 (MQ=255)               gtCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGgtgt                                                                                    <  1:474550/140‑1 (MQ=255)                          cgcgGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCAGTGTTTCTCCAGAtg                                                                         <  1:362860/140‑1 (MQ=255)                           gcgGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGAtgt                                                                        >  1:253245/1‑140 (MQ=255)                              gTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGc                                                                     <  1:175027/140‑1 (MQ=255)                               ttATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGc                                                                     >  1:181875/1‑139 (MQ=255)                               ttATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGc                                                                     <  2:181875/139‑1 (MQ=255)                                    aCAATACGGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCt                                                               <  2:452616/140‑1 (MQ=255)                                      aaTACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGa                                                             <  2:42937/140‑1 (MQ=255)                                                  gcATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGt                                                 >  1:245237/1‑140 (MQ=255)                                                  gcATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGt                                                 >  1:277412/1‑140 (MQ=255)                                                                       aGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTc                            >  2:82194/1‑140 (MQ=255)                                                                            aTAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTc                       <  1:82194/140‑1 (MQ=255)                                                                                       aCCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAg            <  1:261993/140‑1 (MQ=255)                                                                                           gggCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCAc        >  1:320419/1‑140 (MQ=255)                                                                                                 tCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCTCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTaaa  >  2:179733/1‑140 (MQ=255)                                                                                                 tCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCa         >  1:473224/1‑133 (MQ=255)                                                                                                 tCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCa         <  2:473224/133‑1 (MQ=255)                                                                                                        ttCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCtt     <  2:336353/130‑1 (MQ=255)                                                                                                        ttCCACAGGATTCAAAGGAGTGAATGCGGCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCtt     >  1:336353/1‑130 (MQ=255)                                                                                                                                    |                                                                                                         TTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAA  >  NC_000913/950935‑951170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑