Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	979,231	A→G	H969R (CAC→CGC)	mukB →	chromosome partitioning protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	979,231	0	A	G	100.0%	98.8 / NA	28	H969R (CAC→CGC)	mukB	chromosome partitioning protein MukB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/14);  total (14/14)

ATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGAAAAACTGCGTGAACGTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACG  >  NC_000913/979098‑979356                                                                                                                                      |                                                                                                                              aTTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAg                                                                                                                         >  1:322998/1‑140 (MQ=255)  ttGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGc                                                                                                                        <  2:322998/140‑1 (MQ=255)          gTATTGCAGAGCGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGa                                                                                                                <  2:409973/140‑1 (MQ=255)                     cGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGaa                                                                                                      >  2:443035/1‑139 (MQ=255)                     cGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGaa                                                                                                      <  1:443035/139‑1 (MQ=255)                             aaCAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGc                                                                                             >  1:195021/1‑140 (MQ=255)                               cAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCgg                                                                                           <  2:271864/140‑1 (MQ=255)                                aGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGt                                                                                          <  2:204587/140‑1 (MQ=255)                                  ttCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAg                                                                                              <  1:169535/134‑1 (MQ=255)                                  ttCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAg                                                                                              >  2:169535/1‑134 (MQ=255)                                           ttAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGAtct                                                                               >  2:376127/1‑140 (MQ=255)                                            tAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGAtctc                                                                              >  1:387017/1‑140 (MQ=255)                                            tAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGAtctc                                                                              >  2:530959/1‑140 (MQ=255)                                                     aTTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGTGGTAACAGCGATCTCAACGaaaaa                                                                     <  2:280885/140‑1 (MQ=255)                                                     aTTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGAt                                                                                 >  1:439442/1‑128 (MQ=255)                                                     aTTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGAt                                                                                 >  1:472569/1‑128 (MQ=255)                                                     aTTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGAt                                                                                 <  2:439442/128‑1 (MQ=255)                                                     aTTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGaaaaa                                                                     >  2:309732/1‑140 (MQ=255)                                                     aTTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGaaaaa                                                                     >  2:363228/1‑140 (MQ=255)                                                     aTTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCCTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGAt                                                                                 <  2:472569/128‑1 (MQ=255)                                                             tACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGAAAAACTGCGTGa                                                             <  1:506279/140‑1 (MQ=255)                                                              aCTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGAAAAACTGTGTGaa                                                            <  1:376127/140‑1 (MQ=255)                                                                         aTGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGAAAAACTGCGTGAACGTCTGGAACa                                                 >  2:435780/1‑140 (MQ=255)                                                                                 cgATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGAAAAACTGCGTGAACGTCTGGAACAGGCGGAAg                                         <  1:253451/140‑1 (MQ=255)                                                                                   aTGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGAAAAACTGCGTGAACGTCTGGAACAGGCGGAAGCg                                       <  1:363228/140‑1 (MQ=255)                                                                                   aTGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGAAAAACTGCGTGAACGTCTGGAACAGGCGGAAGCg                                       >  2:174220/1‑140 (MQ=255)                                                                                    tGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGAAAAACTGCGTGAACGTCTGGAACAGGCGGAAGCgg                                      >  2:91692/1‑140 (MQ=255)                                                                                                                        gCAGCGTCGTGCGCGCTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGAAAAACTGCGTGAACGTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCAcg  <  1:309732/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                              ATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTATTCTGACTCGGCAGAAATGCTTAGCGGTAACAGCGATCTCAACGAAAAACTGCGTGAACGTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACG  >  NC_000913/979098‑979356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑