Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,064,792	G→A	A253T (GCT→ACT)	yccE →	uncharacterized protein YccE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,064,792	0	G	A	100.0%	144.8 / NA	43	A253T (GCT→ACT)	yccE	uncharacterized protein YccE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/17);  total (26/17)

CGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAG  >  NC_000913/1064659‑1064904                                                                                                                                      |                                                                                                                 cGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCttg                                                                                                            >  2:56021/1‑140 (MQ=255)    tGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCttgttg                                                                                                         >  1:34120/1‑140 (MQ=255)    tGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCttgttg                                                                                                         >  2:427541/1‑140 (MQ=255)       atatTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATa                                                                                                      <  2:369933/140‑1 (MQ=255)           tGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGc                                                                                                  <  2:34120/140‑1 (MQ=255)               tGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTc                                                                                              >  1:395609/1‑140 (MQ=255)                gTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCt                                                                                             >  1:155282/1‑140 (MQ=255)                          ataCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAg                                                                                   <  1:427541/140‑1 (MQ=255)                           taCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGc                                                                                                  >  2:210076/1‑124 (MQ=255)                           taCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGc                                                                                                  <  1:210076/124‑1 (MQ=255)                           taCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAAt                                                                                        >  1:114359/1‑134 (MQ=255)                           taCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAAt                                                                                        <  2:114359/134‑1 (MQ=255)                           taCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAgg                                                                                  >  1:485776/1‑140 (MQ=255)                            aCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAggg                                                                                 <  2:261137/140‑1 (MQ=255)                            aCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAggg                                                                                 <  2:155282/140‑1 (MQ=255)                                gTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGa                                                                             >  2:240354/1‑140 (MQ=255)                                           acaTGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGt                                                                  >  1:51918/1‑140 (MQ=255)                                           acaTGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGt                                                                  >  1:243288/1‑140 (MQ=255)                                            caTGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGtt                                                                 >  1:538583/1‑140 (MQ=255)                                                    ttCATGGGCTCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTAAAATCTCAGGGAAGATGTAAAGACGTTATTTgggg                                                         <  2:192024/140‑3 (MQ=255)                                                    ttCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCg                                                         <  2:243288/140‑1 (MQ=255)                                                      cATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGtt                                                       <  2:20154/140‑1 (MQ=255)                                                         gggCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGAt                                                    >  2:421478/1‑140 (MQ=255)                                                         gggCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGAt                                                    <  2:485776/140‑1 (MQ=255)                                                          ggCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATc                                                   >  1:289550/1‑140 (MQ=255)                                                            cgcTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTa                                                 >  2:64842/1‑140 (MQ=255)                                                            cgcTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTa                                                 >  2:322776/1‑140 (MQ=255)                                                              cTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACa                                               >  2:264176/1‑140 (MQ=255)                                                              cTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACa                                               >  1:267822/1‑140 (MQ=255)                                                                    aGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGa                                         <  1:240354/140‑1 (MQ=255)                                                                               gCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTc                              <  2:117965/140‑1 (MQ=255)                                                                                cAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCa                             >  2:16657/1‑140 (MQ=255)                                                                                    aGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAgggg                         <  2:289550/140‑1 (MQ=255)                                                                                    aGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAgggg                         <  2:320376/140‑1 (MQ=255)                                                                                       aaGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCag                      >  1:318193/1‑140 (MQ=255)                                                                                         gTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCagag                    <  1:264176/140‑1 (MQ=255)                                                                                           ttACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGagta                  >  2:56708/1‑140 (MQ=255)                                                                                                 cGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTa            <  2:259941/140‑1 (MQ=255)                                                                                                   aaTTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATg          >  2:474587/1‑140 (MQ=255)                                                                                                    aTTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGt         >  1:82431/1‑140 (MQ=255)                                                                                                    aTTCATTAAAATGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGt         >  2:448395/1‑140 (MQ=255)                                                                                                           aaaaTGGTTGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAg  >  2:198868/1‑140 (MQ=255)                                                                                                                  ttGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTa            >  2:60532/1‑123 (MQ=255)                                                                                                                  ttGATAAAAAAACGATCGAGACTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTa            <  1:60532/123‑1 (MQ=255)                                                                                                                                      |                                                                                                                 CGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAG  >  NC_000913/1064659‑1064904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑