Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,129,031	A→G	N398S (AAC→AGC)	murJ →	putative lipid II flippase MurJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,129,031	0	A	G	100.0%	77.9 / NA	22	N398S (AAC→AGC)	murJ	putative lipid II flippase MurJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/9);  total (13/9)

ACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGAT  >  NC_000913/1128908‑1129163                                                                                                                            |                                                                                                                                     aCTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGt                                                                                                                      <  1:388494/140‑1 (MQ=255)  cTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTc                                                                                                                     >  1:293538/1‑140 (MQ=255)             tGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACAt                                                                                                          >  1:116522/1‑140 (MQ=255)                   gCCTGTTTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGATAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCggg                                                                                                    <  1:16261/140‑1 (MQ=255)                   gCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCggg                                                                                                    >  1:480294/1‑140 (MQ=255)                      tGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTg                                                                                                 <  2:116522/140‑1 (MQ=255)                                  aaGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTAtt                                                                                     >  1:308277/1‑140 (MQ=255)                                             ccTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTggcggc                                                                          <  2:399111/140‑1 (MQ=255)                                                      tATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGaa                                                                 >  2:114839/1‑140 (MQ=255)                                                           ccGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCtt                                                            <  2:118619/140‑1 (MQ=255)                                                            cGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTc                                                           <  1:114839/140‑1 (MQ=255)                                                                 ggACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCgctgc                                                      <  2:293538/140‑1 (MQ=255)                                                                            cGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAg                                           >  2:317549/1‑140 (MQ=255)                                                                             gCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGt                                          >  1:72961/1‑140 (MQ=255)                                                                              ccAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGtt                                         >  2:233191/1‑140 (MQ=255)                                                                               cAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTg                                        >  2:293776/1‑140 (MQ=255)                                                                                          gCCATCGTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGaa                             <  2:308277/140‑1 (MQ=255)                                                                                                gTTACGCTGATTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCtt                       <  1:317549/140‑1 (MQ=255)                                                                                                         aTTTTAACGCAATTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCa              >  2:104718/1‑140 (MQ=255)                                                                                                                   aaTTGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTgg    >  1:537299/1‑140 (MQ=255)                                                                                                                     ttGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGAt  >  1:95955/1‑140 (MQ=255)                                                                                                                     ttGATGAGCCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGAt  >  2:88601/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                     ACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGAT  >  NC_000913/1128908‑1129163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑