Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,174,594	A→G	V191A (GTG→GCG)	ycfT ←	inner membrane protein YcfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,594	0	A	G	100.0%	55.2 / NA	16	V191A (GTG→GCG)	ycfT	inner membrane protein YcfT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/4);  total (12/4)

CCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCA  >  NC_000913/1174461‑1174722                                                                                                                                      |                                                                                                                                 ccAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCgcac                                                                                                                               >  1:323978/1‑137 (MQ=255) ccAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCgcac                                                                                                                               <  2:323978/137‑1 (MQ=255)    ggCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCa                                                                                                                         >  1:216465/1‑140 (MQ=255)    ggCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCa                                                                                                                         <  2:383465/140‑1 (MQ=255)    ggCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCa                                                                                                                         <  2:216465/140‑1 (MQ=255)        ccgaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCATTcc                                                                                                                     <  2:57355/140‑1 (MQ=255)         cgaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCATTccc                                                                                                                    >  1:42688/1‑140 (MQ=255)           accgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCAtt                                                                                                                       >  2:453830/1‑135 (MQ=255)           accgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCAtt                                                                                                                       <  1:453830/135‑1 (MQ=255)                                  agCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTaa                                                                                           >  2:138641/1‑140 (MQ=255)                                      aTCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGc                                                                                       >  1:360586/1‑140 (MQ=255)                                                ggcggcGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcagc                                                                             >  1:538928/1‑140 (MQ=255)                                                                             gTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAgcgc                                                >  1:217988/1‑140 (MQ=255)                                                                                        ggcgcAAAACCTTGCGCAAAGGCTGTAAAAAGGAAAATTGCGTATCCCACTTTTCCTCCCCCACCACGGGGGGGAAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaa                                     <  2:538928/139‑1 (MQ=255)                                                                                         gcgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaa                                    >  1:167217/1‑140 (MQ=255)                                                                                          cgcAAAACCATGCGCCAAGGCTGTAATAAGCAAAATGGCGGACCCCACTGTTCATCCCCCCCCACGGCGGGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaaa                                   <  2:360586/140‑1 (MQ=255)                                                                                                      ccccAGGGCTGAAATAAGGCAATTTGCGGAGCGCATTTTTCACCCCCCCCCGCGGCGTGGAAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATAcc                       <  2:167217/138‑1 (MQ=255)                                                                                                         cAAGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCATTCCCCACCACGGCGTGGGAATGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACg                    >  1:71144/1‑140 (MQ=255)                                                                                                           aGGCTGTAATAAGGCAAATTGCGGATCGCACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGaa                  >  1:363829/1‑140 (MQ=255)                                                                                                                        gCAAATTGCGGATCGCACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAAc     >  1:209838/1‑140 (MQ=255)                                                                                                                           aaTTGCGGATCGCACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCa  >  1:142024/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                 CCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCA  >  NC_000913/1174461‑1174722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑