Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,271,762	A→G	W30R (TGG→CGG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,271,762	0	A	G	100.0%	54.9 / NA	16	W30R (TGG→CGG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/10);  total (6/10)

AACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAA  >  NC_000913/1271630‑1271890                                                                                                                                     |                                                                                                                                 aaCCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAAc                                                                                                                           >  1:523695/1‑140 (MQ=255)     gTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAACAGCa                                                                                                                       <  1:10143/140‑1 (MQ=255)                                   gTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGTTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCg                                                                                         >  1:18521/1‑140 (MQ=255)                                    tCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGa                                                                                        >  1:286759/1‑140 (MQ=255)                                         aTGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGtct                                                                                   >  1:143080/1‑140 (MQ=255)                                          tGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGtctc                                                                                  <  2:388951/140‑1 (MQ=255)                                                 caacaCTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTgg                                                                           <  2:286759/140‑1 (MQ=255)                                                             cgcTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTAcc                                                               <  2:197676/140‑1 (MQ=255)                                                              gcTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGgttttttcc                                                              >  2:97345/1‑136 (MQ=255)                                                                           ccAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCAtt                                                 <  1:466463/140‑1 (MQ=255)                                                                            cAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCAttt                                                <  2:143080/140‑1 (MQ=255)                                                                              aTAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGa                                              <  1:110883/140‑1 (MQ=255)                                                                                                                   gtgtCTGGCTGTTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGaaa         <  2:18521/140‑1 (MQ=255)                                                                                                                   gtgtCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGaaa         <  1:175833/140‑1 (MQ=255)                                                                                                                   gtgtCTGGCTGCTTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGaaa         <  2:480687/140‑1 (MQ=255)                                                                                                                          gctgctTCCCCGCAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTCCGCCCCTTGTGCATTTTACATTATTGTTTTTCCCTTTCCGATGAATTTATTGAAAAAGTaaa  >  2:175833/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                 AACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAA  >  NC_000913/1271630‑1271890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑