Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,680	A→G	V280A (GTT→GCT)	sapC ←	putrescine ABC exporter membrane protein SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,680	0	A	G	100.0%	67.1 / NA	20	V280A (GTT→GCT)	sapC	putrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/8);  total (12/8)

ATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAG  >  NC_000913/1353546‑1353810                                                                                                                                       |                                                                                                                                   aTACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCcaac                                                                                                                               >  1:191452/1‑140 (MQ=255)       aCCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACg                                                                                                                         <  2:3329/140‑1 (MQ=255)         ccGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCt                                                                                                                       >  2:299894/1‑140 (MQ=255)                aCGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATa                                                                                                                <  1:299894/140‑1 (MQ=255)                              cTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCt                                                                                                    >  2:539693/1‑138 (MQ=255)                              cTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCt                                                                                                    <  1:539693/138‑1 (MQ=255)                                   caCCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGgcagca                                                                                             >  2:457820/1‑140 (MQ=255)                                                   aaTGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGc                                                                             >  1:412533/1‑140 (MQ=255)                                                                    tatCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTTATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCa                                                            <  2:524682/140‑1 (MQ=255)                                                                      tCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAgc                                                          >  2:261626/1‑140 (MQ=255)                                                                             aTGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCAc                                                   >  2:128661/1‑140 (MQ=255)                                                                                 cATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAg                                               <  1:457820/140‑1 (MQ=255)                                                                                      ttCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCg                                          <  1:470040/140‑1 (MQ=255)                                                                                           cccccGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTccc                                     >  2:212158/1‑140 (MQ=255)                                                                                              ccGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCAGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCAt                                  >  2:469327/1‑140 (MQ=255)                                                                                                  aataatCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAg                              <  1:261626/140‑1 (MQ=255)                                                                                                                  aCGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTgc              >  1:316376/1‑140 (MQ=255)                                                                                                                   cGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTgcg             >  2:140652/1‑140 (MQ=255)                                                                                                                          gCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGAt      <  2:316376/140‑1 (MQ=255)                                                                                                                              aggaggTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCgag  >  2:443020/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                   ATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAG  >  NC_000913/1353546‑1353810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑