Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,410,560	A→G	A350A (GCA→GCG)	dbpA →	ATP‑dependent RNA helicase DbpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,410,560	0	A	G	100.0%	85.5 / NA	25	A350A (GCA→GCG)	dbpA	ATP‑dependent RNA helicase DbpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/11);  total (14/11)

ATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCACAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATGGCGGGAAAAAAGCCAAAAT  >  NC_000913/1410434‑1410694                                                                                                                               |                                                                                                                                       aTCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAata                                                                                                                           >  1:183091/1‑140 (MQ=255)  tCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAatat                                                                                                                          <  1:286165/140‑1 (MQ=255)   cGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGcc                                                                                                                               <  1:209375/134‑1 (MQ=255)   cGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGcc                                                                                                                               >  2:209375/1‑134 (MQ=255)   cGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATc                                                                                                                         <  1:505390/140‑1 (MQ=255)     cTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCAt                                                                                                                       >  2:417563/1‑140 (MQ=255)            tggtggTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGAc                                                                                                                <  2:519139/140‑1 (MQ=255)              gtggtgAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACAt                                                                                                              >  2:213008/1‑140 (MQ=255)                                 cGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAAc                                                                                           >  1:348601/1‑140 (MQ=255)                                                   aTGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCt                                                                         >  2:495502/1‑140 (MQ=255)                                                                  gTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCg                                                          >  2:326882/1‑140 (MQ=255)                                                                               tGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAg                                             >  2:103848/1‑140 (MQ=255)                                                                                gCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGaa                                                 >  2:423012/1‑135 (MQ=255)                                                                                gCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGaa                                                 <  1:423012/135‑1 (MQ=255)                                                                                       aTAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCa                                     <  2:279124/140‑1 (MQ=255)                                                                                          gCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACg                                  <  2:85426/140‑1 (MQ=255)                                                                                          gCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACg                                  <  2:94034/140‑1 (MQ=255)                                                                                                            tCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATGGCggg                >  2:176383/1‑140 (MQ=255)                                                                                                            tCTGTGCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGAAACGTTGTGTATCGATGGCggg                >  1:319699/1‑140 (MQ=255)                                                                                                                tgCTCCGGAAGAAGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATGGCGGGaaaa            >  1:112178/1‑140 (MQ=255)                                                                                                                       gaagaaGCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATGGCGGGAAAAAAGCCaa     >  2:113300/1‑140 (MQ=255)                                                                                                                         agaagCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATGGCGGGAAAAAAGCCaaaa   <  2:450570/140‑1 (MQ=255)                                                                                                                          gaagCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATgg                    >  2:292445/1‑122 (MQ=255)                                                                                                                          gaagCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATgg                    <  1:292445/122‑1 (MQ=255)                                                                                                                          gaagCGCAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATGGCGGGAAAAAAGCCAAAAt  <  1:176383/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                       ATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCACAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATGGCGGGAAAAAAGCCAAAAT  >  NC_000913/1410434‑1410694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑