Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,437,610	A→G	Y1059H (TAT→CAT)	ydbK ←	putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,437,610	0	A	G	100.0%	138.5 / NA	39	Y1059H (TAT→CAT)	ydbK	putative pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/15);  total (24/15)

GATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATACGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCC  >  NC_000913/1437484‑1437745                                                                                                                               |                                                                                                                                        gATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCgcttccgcttcc                                                                                                                            >  2:84055/1‑140 (MQ=255)              taGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCa                                                                                                               <  2:529984/140‑1 (MQ=255)                 cGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCg                                                                                                            <  1:70248/140‑1 (MQ=255)                      aGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTgg                                                                                                       >  1:502655/1‑140 (MQ=255)                          gCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCa                                                                                                   >  2:318664/1‑140 (MQ=255)                              gTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTg                                                                                               >  1:480035/1‑140 (MQ=255)                                           ggCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGaa                                                                                  >  2:192499/1‑140 (MQ=255)                                            gcgcATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGaaa                                                                                 >  1:315714/1‑140 (MQ=255)                                                  cTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTgcg                                                                           <  1:90544/140‑1 (MQ=255)                                                  cTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTgcg                                                                           <  1:192499/140‑1 (MQ=255)                                                     gTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCca                                                                        >  2:457761/1‑140 (MQ=255)                                                      tCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCcac                                                                       <  2:268391/140‑1 (MQ=255)                                                          ggCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATaa                                                                   >  1:175872/1‑140 (MQ=255)                                                             tGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACa                                                                <  2:264982/140‑1 (MQ=255)                                                               aGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATg                                                              >  1:217208/1‑140 (MQ=255)                                                               aGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCACTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGcc                                                                         >  2:183350/1‑129 (MQ=255)                                                               aGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCACTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGcc                                                                         <  1:183350/129‑1 (MQ=255)                                                                gTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGa                                                             <  1:318664/140‑1 (MQ=255)                                                                gTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGa                                                             <  2:54001/140‑1 (MQ=255)                                                                 tGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGAc                                                            <  1:146054/140‑1 (MQ=255)                                                                    cAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGt                                                         >  2:105897/1‑140 (MQ=255)                                                                     aGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTa                                                        <  2:315714/140‑1 (MQ=255)                                                                     aGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCAACATAAACATGACCGTa                                                        <  2:502655/140‑1 (MQ=255)                                                                           tAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTAcatcat                                                  >  2:292592/1‑140 (MQ=255)                                                                           tAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTAcatcat                                                  >  1:20809/1‑140 (MQ=255)                                                                                  gCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTg                                           >  1:374540/1‑140 (MQ=255)                                                                                    gctcttcacGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGAc                                         >  1:396491/7‑140 (MQ=255)                                                                                         acacGGGCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCaa                                    <  1:84055/140‑1 (MQ=255)                                                                                             gggCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACTTAAACATGACCGTACATCATCATACTGACGCCAAGATc                                >  2:452970/1‑140 (MQ=255)                                                                                               gCTATAAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCtt                              >  2:88832/1‑140 (MQ=255)                                                                                                  ataAGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTAc                           <  1:105897/140‑1 (MQ=255)                                                                                                     aGCAATGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTAcgcg                        >  1:266067/1‑140 (MQ=255)                                                                                                         aTGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTAcg                          <  2:188061/134‑1 (MQ=255)                                                                                                         aTGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTAcg                          >  1:188061/1‑134 (MQ=255)                                                                                                         aTGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCttt                    >  2:440120/1‑140 (MQ=255)                                                                                                         aTGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCttt                    >  1:226129/1‑140 (MQ=255)                                                                                                         aTGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCttt                    >  2:348667/1‑140 (MQ=255)                                                                                                          tGATCAGCGATGGCCCCGGATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTa                   >  2:213184/1‑140 (MQ=255)                                                                                                                           ggATGCGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGcc  >  2:153101/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                        GATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTCTTCACACGGGCTATAAGCAATGATCAGCGATGGCCCCGGATACGCTTCCGCTTCCTGAATCGCTTTCACCGTCTGGTTCAGCTGCGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCC  >  NC_000913/1437484‑1437745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑