Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,452,470	T→C	V391V (GTA→GTG)	paaZ ←	oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,452,470	0	T	C	100.0%	70.1 / NA	21	V391V (GTA→GTG)	paaZ	oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/6);  total (15/6)

CGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTGCAGCCAGCAATATGTTCACTTTT  >  NC_000913/1452338‑1452599                                                                                                                                     |                                                                                                                                  cgcAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCAccgccg                                                                                                                            >  2:47048/1‑140 (MQ=255)                            cGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCg                                                                                                          >  1:271449/1‑131 (MQ=255)                            cGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCg                                                                                                          <  2:271449/131‑1 (MQ=255)                            cGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACa                                                                                                 >  2:166257/1‑140 (MQ=255)                                      aGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTgg                                                                                       >  1:188046/1‑140 (MQ=255)                                                   cTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCa                                                                          >  2:351335/1‑140 (MQ=255)                                                         aaGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGTTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCgca                                                                    <  1:438614/140‑1 (MQ=255)                                                              aGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGa                                                               <  1:490448/140‑1 (MQ=255)                                                                  gCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATaaa                                                           <  1:298675/140‑1 (MQ=255)                                                                           gTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGa                                                  >  2:375843/1‑140 (MQ=255)                                                                                            ggCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAAtctc                                 >  2:159195/1‑140 (MQ=255)                                                                                                   gCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCt                          <  1:166257/140‑1 (MQ=255)                                                                                                       tGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTGcag                      >  2:481536/1‑140 (MQ=255)                                                                                                       tGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTGcag                      >  2:422/1‑140 (MQ=255)                                                                                                       tGCGACAGGGCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTAcag                      >  2:34563/1‑140 (MQ=255)                                                                                                              gggCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTGcagc                     <  2:308929/134‑1 (MQ=255)                                                                                                              gggCCAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTGcagc                     >  1:308929/1‑134 (MQ=255)                                                                                                                 ccAAAGGCTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTGCAGCCAGCAatat            >  1:294317/1‑140 (MQ=255)                                                                                                                        cTTCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTGCAGCCAGCAATATGTTCACt     >  1:277561/1‑140 (MQ=255)                                                                                                                          tCTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTGCAGCCAGCAATATGTTCACttt   >  2:471281/1‑140 (MQ=255)                                                                                                                           cTGTTGCATGCACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTGCAGCCAGCAATATGTTCACtttt  >  2:161/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                  CGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCCGCCTGACCACCGAGGCGAATCTCGCATCCTGCAGCCAGCAATATGTTCACTTTT  >  NC_000913/1452338‑1452599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑