Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,539,368	G→A	Q1075* (CAG→TAG)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,539,368	0	G	A	100.0%	88.2 / NA	26	Q1075* (CAG→TAG)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/15);  total (11/15)

CTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATG  >  NC_000913/1539232‑1539503                                                                                                                                         |                                                                                                                                        cTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAgcg                                                                                                                                      <  2:521190/140‑1 (MQ=255)   gATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCa                                                                                                                                    <  2:481296/140‑1 (MQ=255)   gATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCa                                                                                                                                    >  1:481296/1‑140 (MQ=255)    aTCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCAt                                                                                                                                   <  2:115980/140‑1 (MQ=255)              tCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACg                                                                                                                         <  2:461510/140‑1 (MQ=255)                ggTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCt                                                                                                                       <  2:348143/140‑1 (MQ=255)                          gagagaGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTC‑GGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGa                                                                                                            <  1:319680/140‑1 (MQ=255)                                                          tAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCtt                                                                                  >  1:290164/1‑135 (MQ=255)                                                          tAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCtt                                                                                  <  2:290164/135‑1 (MQ=255)                                                          tAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCAcc                                                                             >  1:341893/1‑140 (MQ=255)                                                          tAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTAAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCAcc                                                                             >  1:354928/1‑140 (MQ=255)                                                             gTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCaaa                                                                          >  1:65628/1‑140 (MQ=255)                                                              ttGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCaaaa                                                                         >  2:127405/1‑140 (MQ=255)                                                                    gAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGc                                                                   >  2:426604/1‑140 (MQ=255)                                                                      aTGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCTATAAGCCACCAGGCTTTCACCAAAAGCACGCAt                                                                 >  1:274782/1‑140 (MQ=255)                                                                       tGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATc                                                                >  2:396186/1‑140 (MQ=255)                                                                              tttCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGgat                                                         <  1:396186/140‑1 (MQ=255)                                                                                         gcgTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATag                                              >  1:406341/1‑140 (MQ=255)                                                                                         gcgTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATag                                              >  1:323722/1‑140 (MQ=255)                                                                                                gAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAgctgct                                       <  2:111/140‑1 (MQ=255)                                                                                                    ttAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCg                                   <  2:535680/140‑1 (MQ=255)                                                                                                       aGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTcc                                <  2:406341/140‑1 (MQ=255)                                                                                                        gTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTccc                               <  2:65628/140‑1 (MQ=255)                                                                                                                              tGGCGGTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTa         <  1:535187/140‑1 (MQ=255)                                                                                                                                  ggTATCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTc     <  2:502512/140‑1 (MQ=255)                                                                                                                                     aTCTAGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATg  <  2:489451/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATG  >  NC_000913/1539232‑1539503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑