Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,559,278	T→C	L211L (CTA→CTG)	ddpC ←	putative D,D‑dipeptide ABC transporter membrane subunit DdpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,559,278	0	T	C	100.0%	74.5 / NA	22	L211L (CTA→CTG)	ddpC	putative D,D‑dipeptide ABC transporter membrane subunit DdpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

ACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCG  >  NC_000913/1559152‑1559412                                                                                                                               |                                                                                                                                       aCCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCTAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTa                                                                                                                           >  2:365006/1‑140 (MQ=255) aCCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTa                                                                                                                           >  1:333194/1‑140 (MQ=255) aCCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGCGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTa                                                                                                                           >  1:85047/1‑140 (MQ=255)       tGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAatat                                                                                                                     <  1:337185/140‑1 (MQ=255)              aTCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGtt                                                                                                              <  2:342059/140‑1 (MQ=255)                     cccATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGa                                                                                                       <  2:411330/140‑1 (MQ=255)                                  cGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGAcg                                                                                          <  1:117759/140‑1 (MQ=255)                                   ggTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGAcgc                                                                                         >  1:7376/1‑140 (MQ=255)                                                  aGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTcgc                                                                          >  1:427626/1‑140 (MQ=255)                                                     ccAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTcgccgc                                                                       <  2:333194/140‑1 (MQ=255)                                                        aTAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTg                                                                    <  2:85047/140‑1 (MQ=255)                                                          aaaTCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGaa                                                                  <  2:530056/140‑1 (MQ=255)                                                          aaaTCCCAACGTGGCGGCCATTAAAATCGCGATACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGaa                                                                  <  2:348323/140‑1 (MQ=255)                                                                 aaCGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGt                                                           >  1:327722/1‑140 (MQ=255)                                                                  aCGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAAc                                                                 <  2:91457/133‑1 (MQ=255)                                                                  aCGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAAc                                                                 >  1:91457/1‑133 (MQ=255)                                                                       gcggcCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGT‑AAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTgg                                                    >  1:442749/1‑140 (MQ=255)                                                                            cATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTa                                                <  2:234002/140‑1 (MQ=255)                                                                                     cgcgCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATg                                       >  2:286500/1‑140 (MQ=255)                                                                                                  tCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCgcgc                          <  1:286500/140‑1 (MQ=255)                                                                                                       cGATGCCTGCACGATCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGgc                     >  1:274157/1‑140 (MQ=255)                                                                                                                     tCAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGCTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGa       >  1:106259/1‑140 (MQ=255)                                                                                                                      cAGCGGCGGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGaa      >  2:298702/1‑140 (MQ=255)                                                                                                                        gcggcgGCAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAAtt    <  2:106259/140‑1 (MQ=255)                                                                                                                          ggcggcAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCg  <  2:276321/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                       ACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCG  >  NC_000913/1559152‑1559412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑