Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,578,090 G→A P418L (CCG→CTG)  yddA ← ABC transporter family protein YddA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,578,0900GA100.0% 142.1 / NA 41P418L (CCG→CTG) yddAABC transporter family protein YddA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/22);  total (19/22)

CGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACGGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGTTCTCTAATATGATCTTATTATCAGGCGTA  >  NC_000913/1577956‑1578218
                                                                                                                                      |                                                                                                                                
cGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCcag                                                                                                                             >  1:476242/1‑140 (MQ=255)
   tCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCcagcag                                                                                                                          >  1:500112/1‑140 (MQ=255)
          tttATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATa                                                                                                                   <  2:142948/140‑1 (MQ=255)
                   tACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTaa                                                                                                          >  1:490198/1‑140 (MQ=255)
                    aCGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAg                                                                                                         <  2:97634/140‑1 (MQ=255)
                           ggggAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGtgtg                                                                                                  >  2:529449/1‑140 (MQ=255)
                            gggAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATa                                                                                                                   <  1:278809/122‑1 (MQ=255)
                            gggAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATa                                                                                                                   >  2:278809/1‑122 (MQ=255)
                            gggAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTgg                                                                                                 >  1:251448/1‑140 (MQ=255)
                            gggAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTgg                                                                                                 >  1:454668/1‑140 (MQ=255)
                              gAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGtt                                                                                               <  2:476242/140‑1 (MQ=255)
                              gAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGtt                                                                                               >  1:468825/1‑140 (MQ=255)
                                     tttACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCcg                                                                                        <  2:490198/140‑1 (MQ=255)
                                         cAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGcc                                                                                    >  1:119715/1‑140 (MQ=255)
                                            ataatCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCaga                                                                                 >  2:149857/1‑140 (MQ=255)
                                             taatCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCagag                                                                                <  2:278036/140‑1 (MQ=255)
                                             taatCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCagaa                                                                                <  2:119715/140‑2 (MQ=255)
                                               atCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTa                                                                              <  2:500112/140‑1 (MQ=255)
                                                tctcTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAg                                                                             <  2:500501/140‑1 (MQ=255)
                                                   ctTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCt                                                                          >  2:180220/1‑140 (MQ=255)
                                                    tttCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCtt                                                                         <  2:454668/140‑1 (MQ=255)
                                                     ttCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCttt                                                                        <  2:468825/140‑1 (MQ=255)
                                                     ttCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCttt                                                                        <  2:438546/140‑1 (MQ=255)
                                                      tCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTc                                                                       >  2:485149/1‑140 (MQ=255)
                                                                  ttttGATTAACGGTGTTTGTGACACATACCAACTGTCAGAAGGAGAAGAAATATCTCCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCACGCGCCCGAGTAGCCCTTCAGTAATAGCCAt                                                           <  2:76733/140‑1 (MQ=255)
                                                                           aCGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGc                                                  >  1:370772/1‑140 (MQ=255)
                                                                               tgtTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGaaa                                              <  2:370772/140‑1 (MQ=255)
                                                                                             aCCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAgg                                <  2:285330/140‑1 (MQ=255)
                                                                                             aCCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAgg                                <  1:529449/140‑1 (MQ=255)
                                                                                              ccAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGt                               <  2:298031/140‑1 (MQ=255)
                                                                                               cAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGtt                              <  1:149857/140‑1 (MQ=255)
                                                                                                  cTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGTtctc                           >  1:101141/1‑140 (MQ=255)
                                                                                                     tCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGa                                         <  1:96007/123‑1 (MQ=255)
                                                                                                     tCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGa                                         >  2:96007/1‑123 (MQ=255)
                                                                                                     tCAGCAGGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGTTCTCTaa                        >  2:289653/1‑140 (MQ=255)
                                                                                                         cagGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGtt                              >  1:256862/1‑130 (MQ=255)
                                                                                                         cagGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGtt                              <  2:256862/130‑1 (MQ=255)
                                                                                                         cagGAGAAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGTTCTCTAATATg                    <  2:101141/140‑1 (MQ=255)
                                                                                                            gagaAGAAATATCACCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGTTCTCTAATATGATc                 <  2:235738/140‑1 (MQ=255)
                                                                                                                         aCCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGTTCTCTAATATGATCTTATTATCAGGCg    <  2:251448/140‑1 (MQ=255)
                                                                                                                         aCCTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGTTCTCTAATATGATCTTATTATCAGGCg    >  2:188511/1‑140 (MQ=255)
                                                                                                                          ccTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGTTCTCTAATATGATCTTATTATCAGGCGt   >  2:139815/1‑140 (MQ=255)
                                                                                                                           cTTTAAACCACAGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGTTCTCTAATATGATCTTATTATCAGGCGTa  >  1:422472/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                
CGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTAAACCACGGCCAGCAGTGGGATAATGTTTTAAGCAGTGTGGTTTTTCCCGCGCCAGAGTAGCCTTTCAGTAATAGCCATTTGCCTGGCGAAACATGAAAGTTCAGGTTCTCTAATATGATCTTATTATCAGGCGTA  >  NC_000913/1577956‑1578218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑