Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,609,314	A→G	V456A (GTG→GCG)	uxaB ←	tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,609,314	0	A	G	100.0%	212.0 / NA	58	V456A (GTG→GCG)	uxaB	tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/33);  total (25/33)

GGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCT  >  NC_000913/1609180‑1609446                                                                                                                                       |                                                                                                                                     ggTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGc                                                                                                                                 >  1:369643/1‑140 (MQ=255)   tGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGt                                                                                                                               >  2:343290/1‑140 (MQ=255)    gAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTc                                                                                                                              <  1:332816/140‑1 (MQ=255)    gAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTc                                                                                                                              <  1:16872/140‑1 (MQ=255)    gAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTc                                                                                                                              <  2:526548/140‑1 (MQ=255)     aaaTAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGt                                                                                                                               <  2:232079/138‑1 (MQ=255)     aaaTAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGt                                                                                                                               >  1:232079/1‑138 (MQ=255)     aaaTAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCa                                                                                                                             >  2:472698/1‑140 (MQ=255)       aTAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAgg                                                                                                                           >  1:133666/1‑140 (MQ=255)        tAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGt                                                                                                                          <  2:369643/140‑1 (MQ=255)            aaaCCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTg                                                                                                                      >  2:299632/1‑140 (MQ=255)             aaCCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCg                                                                                                                                <  1:298783/129‑1 (MQ=255)             aaCCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCg                                                                                                                                >  2:298783/1‑129 (MQ=255)               cccGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTc                                                                                                                   >  2:282380/1‑140 (MQ=255)                   gTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAg                                                                                                               >  2:123043/1‑140 (MQ=255)                             ggTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCttttt                                                                                                     <  1:472698/140‑1 (MQ=255)                              gTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTc                                                                                                    >  2:216781/1‑140 (MQ=255)                                  aGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGcc                                                                                                >  1:321025/1‑140 (MQ=255)                                            gtgtTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCg                                                                                      <  1:415188/140‑1 (MQ=255)                                            gtgtTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCg                                                                                      <  1:276124/140‑1 (MQ=255)                                                tattaGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTAc                                                                                  <  1:123043/140‑1 (MQ=255)                                                 attaGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTAcc                                                                                 <  1:282380/140‑1 (MQ=255)                                                     gCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTAc                                                                                  <  2:15153/135‑1 (MQ=255)                                                     gCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTAc                                                                                  >  1:15153/1‑135 (MQ=255)                                                       acaACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTcc                                                                           <  1:166644/140‑1 (MQ=255)                                                       acaACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTcc                                                                           >  2:166644/1‑140 (MQ=255)                                                         aaCGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTg                                                                         >  2:490333/1‑140 (MQ=255)                                                          aCGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTgg                                                                        <  2:348430/140‑1 (MQ=255)                                                           cGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTggg                                                                       >  2:391821/1‑140 (MQ=255)                                                           cGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTggg                                                                       >  1:18855/1‑140 (MQ=255)                                                            ggACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGt                                                                      <  2:196669/140‑1 (MQ=255)                                                              aCGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTggg                                                                       <  2:208618/137‑1 (MQ=255)                                                              aCGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTCTCTGCCAAAACAATCGCTACCAGTTCCTggg                                                                       >  1:208618/1‑137 (MQ=255)                                                                    aGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATaa                                                              <  1:343290/140‑1 (MQ=255)                                                                    aGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATaa                                                              <  1:299632/140‑1 (MQ=255)                                                                              aTCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACg                                                    >  1:61319/1‑140 (MQ=255)                                                                                  cTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGc                                                <  2:321025/140‑1 (MQ=255)                                                                                      ttCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGc                                            >  2:86218/1‑140 (MQ=255)                                                                                      ttCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGc                                            <  1:143483/140‑1 (MQ=255)                                                                                        cGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTc                                          <  2:133666/140‑1 (MQ=255)                                                                                          agaATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATg                                                 <  2:418551/131‑1 (MQ=255)                                                                                          agaATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATg                                                 >  1:418551/1‑131 (MQ=255)                                                                                           gaATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCag                                       >  1:533642/1‑140 (MQ=255)                                                                                               cGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGtt                                   <  2:18855/140‑1 (MQ=255)                                                                                                           aTTAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGtt                       <  2:61319/140‑1 (MQ=255)                                                                                                             tAGCAACCTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTcc                     <  1:442860/140‑1 (MQ=255)                                                                                                                    cTGCTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAgt              <  2:533642/140‑1 (MQ=255)                                                                                                                       cTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTccagccag               <  1:192416/136‑1 (MQ=255)                                                                                                                       cTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTccagccag               >  2:192416/1‑136 (MQ=255)                                                                                                                       cTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGtgtg           >  1:499865/1‑140 (MQ=255)                                                                                                                       cTCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGtgtg           >  1:479374/1‑140 (MQ=255)                                                                                                                        tCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTccagc                  >  2:317318/1‑132 (MQ=255)                                                                                                                        tCGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTccagc                  <  1:317318/132‑1 (MQ=255)                                                                                                                         cGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAgtgttca         <  1:391821/140‑4 (MQ=255)                                                                                                                         cGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCa         <  1:513584/140‑1 (MQ=255)                                                                                                                         cGACCAGACCAGGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCa         <  1:86218/140‑1 (MQ=255)                                                                                                                              agaccagGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTc    <  1:216781/140‑1 (MQ=255)                                                                                                                               gaccagGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTAACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTcc   <  1:490333/140‑1 (MQ=255)                                                                                                                                accagGCGCTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCt  <  2:479374/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     GGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCT  >  NC_000913/1609180‑1609446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑