BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	90,891	A→G	L266L (TTA→TTG)	rsmH →	16S rRNA m(4)C1402 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	90,891	0	A	G	100.0%	124.5 / NA	35	L266L (TTA→TTG)	rsmH	16S rRNA m(4)C1402 methyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/17); total (18/17)

GGCGCTAAAAAGCTCGCTCAACGTGCTGGCCCCGGGTGGGCGGCTTTCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTACCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCAGAGAGGACGA  >  NC_000913/90756‑91027

ggCGCTAAAAAGCTCGCTCAACGTGCTGGCCCCGGGTGGGCGGCTTTCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGa                                                                                                                                      <  2:114931/140‑1 (MQ=255)

 gcgcTAAAAAGCTCGCTCAACGTGCTGGCCCCGGGTGGGCGGCTTTCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGAt                                                                                                                                     >  2:368225/1‑140 (MQ=255)

  cgcTAAAAAGCTCGCTCAACGTGCTGGCCCCGGGTGGGCGGCTTTCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATg                                                                                                                                    >  1:108492/1‑140 (MQ=255)

              cgctcAACGTGCTGGCCCCGGGTGGGCGGCTTTCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGagcag                                                                                                                        >  2:359708/1‑140 (MQ=255)

              cgctcAACGTGCTGGCCCCGGGTGGGCGGCTTTCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGagcag                                                                                                                        <  1:501699/140‑1 (MQ=255)

                             ccccGGGTGGGCGGCTTTCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGt                                                                                                         >  2:444581/1‑140 (MQ=255)

                                      ggcggcTTTCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAg                                                                                                <  2:302442/140‑1 (MQ=255)

                                      ggcggcTTTCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAg                                                                                                >  1:59560/1‑140 (MQ=255)

                                             ttCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAg                                                                                         >  1:331268/1‑140 (MQ=255)

                                               cGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCa                                                                                       <  1:444581/140‑1 (MQ=255)

                                                    atcaGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAgg                                                                                  <  1:430389/140‑1 (MQ=255)

                                                     tcaGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAg                                                                                                <  1:11881/125‑1 (MQ=255)

                                                     tcaGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAg                                                                                                >  2:11881/1‑125 (MQ=255)

                                                            ccACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTaa                                                                          >  2:20059/1‑140 (MQ=255)

                                                                  gCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCgg                                                                    >  2:397688/1‑140 (MQ=255)

                                                                  gCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCgg                                                                    <  1:393856/140‑1 (MQ=255)

                                                                            cGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGc                                                                       >  2:178777/1‑127 (MQ=255)

                                                                            cGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGc                                                                       <  1:178777/127‑1 (MQ=255)

                                                                              tATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAgg                                                        >  2:203280/1‑140 (MQ=255)

                                                                                     aaaCGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTga                                                 >  2:369313/1‑140 (MQ=255)

                                                                                       aCGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTgaga                                               <  1:339870/140‑1 (MQ=255)

                                                                                        cGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGaa                                              <  1:397688/140‑1 (MQ=255)

                                                                                          ttttATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAAcc                                            >  2:463895/1‑140 (MQ=255)

                                                                                                   tGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAAcc                                            <  2:13082/131‑1 (MQ=255)

                                                                                                   tGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAAcc                                            >  1:13082/1‑131 (MQ=255)

                                                                                                    gAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCg                                  <  2:108492/140‑1 (MQ=255)

                                                                                                    gAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCg                                  <  1:369313/140‑1 (MQ=255)

                                                                                                              gcgGTCCGCAAGTTCCGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTagttcagtt                        <  1:359708/140‑1 (MQ=255)

                                                                                                                            ccGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt                      >  1:395181/1‑128 (MQ=255)

                                                                                                                            ccGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt                      <  2:395181/128‑1 (MQ=255)

                                                                                                                            ccGGCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCaga          >  2:102246/1‑140 (MQ=255)

                                                                                                                              ggCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt                      <  1:88827/126‑1 (MQ=255)

                                                                                                                              ggCAGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt                      >  2:88827/1‑126 (MQ=255)

                                                                                                                                 aGGGTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCAGAGAGGa     <  2:9077/140‑1 (MQ=255)

                                                                                                                                    gTTGCCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCAGAGAGGACGa  >  1:447865/1‑140 (MQ=255)

GGCGCTAAAAAGCTCGCTCAACGTGCTGGCCCCGGGTGGGCGGCTTTCGATCATCAGCTTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTACCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCAGAGAGGACGA  >  NC_000913/90756‑91027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`