Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,654,770	G→A	T124T (ACC→ACT)	rspA ←	mandelate racemase/muconate lactonizing enzyme family protein RspA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,654,770	0	G	A	100.0%	59.1 / NA	18	T124T (ACC→ACT)	rspA	mandelate racemase/muconate lactonizing enzyme family protein RspA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/11);  total (7/11)

ACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAACCGG  >  NC_000913/1654644‑1654904                                                                                                                               |                                                                                                                                       acctttacctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAAt                                                                                                                           <  1:339686/140‑1 (MQ=255)                                   aTACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAg                                                                                         >  1:172611/1‑140 (MQ=255)                                       cAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGAcg                                                                                                   >  2:319509/1‑126 (MQ=255)                                       cAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGAcg                                                                                                   <  1:319509/126‑1 (MQ=255)                                          ggATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTgg                                                                                  >  1:316760/1‑140 (MQ=255)                                                               tGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAGACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCt                                                             <  2:88218/140‑1 (MQ=255)                                                                 gCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCttt                                                           >  1:480189/1‑140 (MQ=255)                                                                 gCTTGGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAGACCATCACCCCTTCACGAGACGCGCCGCCGAGCAACTGGTAAAGCGGCATGTTCGCAGAttt                                                           >  2:402767/1‑140 (MQ=255)                                                                      gAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCtt                                                      <  1:378906/140‑1 (MQ=255)                                                                                  ttGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCcaca                                          <  1:273703/140‑1 (MQ=255)                                                                                   tgatgaCGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAg                                         >  2:153013/1‑140 (MQ=255)                                                                                             cATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCa                               <  2:316760/140‑1 (MQ=255)                                                                                             cATAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCa                               <  1:225254/140‑1 (MQ=255)                                                                                              aTAATCATCCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCaa                              >  2:530500/1‑140 (MQ=255)                                                                                                    atcCAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTg                        <  2:66747/140‑1 (MQ=255)                                                                                                      ccAGAGCTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGaa                      >  2:276021/1‑140 (MQ=255)                                                                                                            cTTCATCAATACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATAT‑CCACAGCGCCATATCAACCGCTGAAATGGCCg               <  2:172611/140‑1 (MQ=255)                                                                                                                    aTACTGTGACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGt        <  1:276021/140‑1 (MQ=255)                                                                                                                          tgACCAGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAACCgg  <  1:200312/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                       ACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAACCGG  >  NC_000913/1654644‑1654904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑