Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,700,566	G→A	A404A (GCG→GCA)	malX →	PTS enzyme IIBC component MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,700,566	0	G	A	100.0%	70.1 / NA	21	A404A (GCG→GCA)	malX	PTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/8);  total (13/8)

TTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGG  >  NC_000913/1700429‑1700700                                                                                                                                          |                                                                                                                                       ttAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTggcagc                                                                                                                                      >  1:233419/1‑140 (MQ=255)  tAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGgcagca                                                                                                                                     <  1:441706/140‑1 (MQ=255)         ccTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGt                                                                                                                              <  2:233419/140‑1 (MQ=255)         ccTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGt                                                                                                                              >  1:345198/1‑140 (MQ=255)         ccTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGt                                                                                                                              >  1:435290/1‑140 (MQ=255)                              tgtgCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCt                                                                                                         <  2:152772/140‑1 (MQ=255)                                tgCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTc                                                                                                       <  1:325225/140‑1 (MQ=255)                                tgCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTc                                                                                                       <  1:327607/140‑1 (MQ=255)                                    cGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGtt                                                                                                   <  2:254786/140‑1 (MQ=255)                                          cACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGtt                                                                                                   >  1:113043/1‑134 (MQ=255)                                          cACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGtt                                                                                                   <  2:113043/134‑1 (MQ=255)                                                           aCGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTg                                                                            >  2:425520/1‑140 (MQ=255)                                                                 atatCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAAcc                                                                      >  2:126918/1‑140 (MQ=255)                                                                              cGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATa                                                         >  2:439988/1‑140 (MQ=255)                                                                              cGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATa                                                         >  1:350645/1‑140 (MQ=255)                                                                                   tgtTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGaa                                                    <  2:345198/140‑1 (MQ=255)                                                                                                               cAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTg                        >  1:416000/1‑140 (MQ=255)                                                                                                                aaGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGc                       >  1:23578/1‑140 (MQ=255)                                                                                                                    ggTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGt                   >  2:19438/1‑140 (MQ=255)                                                                                                                            gTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCggg           >  1:152120/1‑140 (MQ=255)                                                                                                                               ccAGTGGTGGGAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTaa        >  2:446843/1‑140 (MQ=255)                                                                                                                                 aGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAAt      <  1:392647/140‑1 (MQ=255)                                                                                                                                     gtggCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAgg  >  1:97760/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGG  >  NC_000913/1700429‑1700700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑