Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,506	G→A	P18L (CCG→CTG)	btuD ←	vitamin B12 ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,506	0	G	A	100.0%	49.7 / NA	16	P18L (CCG→CTG)	btuD	vitamin B12 ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/6);  total (10/6)

GTTGCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCG  >  NC_000913/1793375‑1793607                                                                                                                                    |                                                                                                      gTTGCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAgg                                                                                               >  1:501245/1‑140 (MQ=255)               tGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGc                                                                                 >  2:220137/1‑140 (MQ=255)                         ccTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGt                                                                       >  1:315525/1‑140 (MQ=255)                              ccGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACTCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTGGATTCCGCAACATCTTGTAACTg                                                                  <  2:501245/140‑1 (MQ=255)                                gCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCa                                                                <  1:220137/140‑1 (MQ=255)                                              ccATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACatt                                                  >  2:401863/1‑140 (MQ=255)                                                           tAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGc                                     >  1:310742/1‑140 (MQ=255)                                                                       aCCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCt                         >  2:502043/1‑140 (MQ=255)                                                                        cccGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCtt                        <  2:30678/140‑1 (MQ=255)                                                                            cgcCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGGAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCa                    >  1:326575/1‑140 (MQ=255)                                                                             gcCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCAt                   <  2:315525/140‑1 (MQ=255)                                                                               cATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATca                 <  1:502043/140‑1 (MQ=255)                                                                                 ttCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATcaca               >  2:328098/1‑140 (MQ=255)                                                                                         caccaGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATc       >  2:351392/1‑140 (MQ=255)                                                                                           ccaGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCtt     >  2:220476/1‑140 (MQ=255)                                                                                              ggTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCAGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCg  <  1:401863/140‑1 (MQ=255)                                                                                                                                    |                                                                                                      GTTGCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCG  >  NC_000913/1793375‑1793607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑