Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,808,009	A→G	T72A (ACC→GCC)	yniA →	putative kinase YniA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,808,009	0	A	G	100.0%	69.8 / NA	20	T72A (ACC→GCC)	yniA	putative kinase YniA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/10);  total (10/10)

CGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTA  >  NC_000913/1807873‑1808134                                                                                                                                         |                                                                                                                              cGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCg                                                                                                                            <  2:427137/140‑1 (MQ=255)      aaGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCt                                                                                                                       <  2:320559/140‑1 (MQ=255)                           tGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCt                                                                                                  <  2:417745/140‑1 (MQ=255)                           tGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCt                                                                                                  >  2:406202/1‑140 (MQ=255)                            gcgcTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTg                                                                                                 >  1:117782/1‑140 (MQ=255)                               cTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGAcc                                                                                              <  1:163929/140‑1 (MQ=255)                                      ggACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTa                                                                                       <  2:117782/140‑1 (MQ=255)                                         cATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAg                                                                                    >  2:277311/1‑140 (MQ=255)                                             aTTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGtttt                                                                                <  1:252759/140‑1 (MQ=255)                                                       cAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATgg                                                                      >  2:13350/1‑140 (MQ=255)                                                                        aaCTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGt                                                     >  1:424468/1‑140 (MQ=255)                                                                            gCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGc                                                 <  1:277311/140‑1 (MQ=255)                                                                                cccGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGa                                             >  1:462818/1‑140 (MQ=255)                                                                                cccGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGa                                             >  1:446309/1‑140 (MQ=255)                                                                                         aCCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAg                                    <  2:424468/140‑1 (MQ=255)                                                                                                        cAACTGGAGTTACTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGTGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTgg                     <  2:48373/140‑1 (MQ=255)                                                                                                                   aCTGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTTGTCAGCAAATTg          >  2:253937/1‑140 (MQ=255)                                                                                                                     tGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTgc         <  2:410183/139‑1 (MQ=255)                                                                                                                     tGTCGCGTAGTAAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTgc         >  1:410183/1‑139 (MQ=255)                                                                                                                           gtagtaAAACCGTCGCCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTa  >  2:94998/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                              CGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTA  >  NC_000913/1807873‑1808134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑