Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,819,092	T→C	Y119C (TAC→TGC)	chbR ←	DNA‑binding transcriptional dual regulator ChbR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,819,092	0	T	C	100.0%	84.4 / NA	24	Y119C (TAC→TGC)	chbR	DNA‑binding transcriptional dual regulator ChbR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/14);  total (10/14)

GTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATAAAAACTTTGATGGTGCGAACCTAAC  >  NC_000913/1818955‑1819223                                                                                                                                          |                                                                                                                                    gTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGcat                                                                                                                                   >  1:214700/1‑140 (MQ=255)  ttcttcGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGcata                                                                                                                                  >  2:117529/1‑140 (MQ=255)   tcttcGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATAc                                                                                                                                 >  2:179022/1‑140 (MQ=255)                           ttAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAgg                                                                                                         >  2:11925/1‑140 (MQ=255)                                   aTAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATg                                                                                                 <  2:214700/140‑1 (MQ=255)                                   aTAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAAAGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATg                                                                                                 <  1:111923/140‑1 (MQ=255)                                        aaGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAgg                                                                                            <  1:179022/140‑1 (MQ=255)                                                            tctTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCg                                                                        <  2:293401/140‑1 (MQ=255)                                                             ctTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGt                                                                       <  1:428180/140‑1 (MQ=255)                                                                      cTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATc                                                              <  1:262402/140‑1 (MQ=255)                                                                        gTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATccc                                                            >  1:46485/1‑140 (MQ=255)                                                                        gTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATccc                                                            >  2:94715/1‑140 (MQ=255)                                                                              cgGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGtt                                                      >  1:171198/1‑140 (MQ=255)                                                                                         tGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGcgcg                                           <  1:341702/140‑1 (MQ=255)                                                                                                   aCTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCaaa                                 <  1:11925/140‑1 (MQ=255)                                                                                                     tGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACt                               >  1:160553/1‑140 (MQ=255)                                                                                                        ttCCACATAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCAt                            <  1:94715/140‑1 (MQ=255)                                                                                                           cacaTAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATaaa                         <  2:171198/140‑1 (MQ=255)                                                                                                           cacaTAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATaaa                         <  1:117529/140‑1 (MQ=255)                                                                                                            acaTAGGTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATaaaa                        >  1:177533/1‑140 (MQ=255)                                                                                                                 ggTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATAAAAACttt                   >  2:165626/1‑140 (MQ=255)                                                                                                                  gTGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATAAAAACTTTg                  >  1:447601/1‑140 (MQ=255)                                                                                                                   tGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATAAAAACTTTGa                 <  1:505844/140‑1 (MQ=255)                                                                                                                   tGAGAAAAGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATAAAAACTTTGa                 <  2:178305/140‑1 (MQ=255)                                                                                                                       aaaaGCGTTATTGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATAAAAACTTTGATGGt             >  1:38965/1‑140 (MQ=255)                                                                                                                                  tGGTCCGGCATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATAAAAACTTTGATGGTGCGAACCTAAc  <  2:390060/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    GTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCACCAAACTCATAAAAACTTTGATGGTGCGAACCTAAC  >  NC_000913/1818955‑1819223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑