Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	121,462	C→T	G30G (GGG→GGA)	aroP ←	aromatic amino acid:H(+) symporter AroP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	121,462	0	C	T	100.0%	95.5 / NA	28	G30G (GGG→GGA)	aroP	aromatic amino acid:H(+) symporter AroP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/12);  total (16/12)

CGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGC  >  NC_000913/121338‑121595                                                                                                                             |                                                                                                                                      cGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCAccc                                                                                                                        >  1:346311/1‑140 (MQ=255)     caccaTTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAgcg                                                                                                                    >  2:439447/1‑140 (MQ=255)      accaTTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAgcgc                                                                                                                   >  1:8576/1‑140 (MQ=255)       ccaTTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGcgcg                                                                                                                  <  2:442955/140‑1 (MQ=255)                  aGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAat                                                                                                       >  1:414439/1‑140 (MQ=255)                        cGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCg                                                                                                 <  2:248877/140‑1 (MQ=255)                              aTCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGttttt                                                                                           >  1:463273/1‑140 (MQ=255)                                        cGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGGTTTTTAGGCCccgc                                                                                 >  1:278208/1‑140 (MQ=255)                                             aaaCCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAAGCCGCGCctttt                                                                            >  2:123877/1‑138 (MQ=255)                                                                   ggATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCGCGCTTTTGCTGCTCCCCCCGCCGTTGATCt                                                      >  2:65970/1‑140 (MQ=255)                                                                     aTAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTTACCTTc                                                    <  2:406720/140‑1 (MQ=255)                                                                     aTAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGATCTTc                                                    <  1:65970/140‑1 (MQ=255)                                                                     aTAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTc                                                    <  2:463273/140‑1 (MQ=255)                                                                         tCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAAGCCGCGCTTTTGCTGCTCGCCCGGCTGTTGACCTTCcctc                                                >  2:480502/1‑140 (MQ=255)                                                                            cTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATg                                             <  2:439425/140‑1 (MQ=255)                                                                                 ccTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAAcc                                        <  1:233055/140‑1 (MQ=255)                                                                                    gCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCGCGCCTTAGCTGCTCGCCGTGCTGTTGTCCTTCCCTCATTAAAACTcc                                     >  2:330984/1‑139 (MQ=255)                                                                                    gCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAAGCCCCGCCTTTGCTGCTCGCCGTGCTGTTGTCCTTCCCTCATTAAACCTCg                                     >  2:233055/1‑140 (MQ=255)                                                                                         cTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCGCGGTTTTGCTGCTCGCCCGGCTGTTGACCTTCCCTCATGAAAACTCGTgggg                                >  2:329060/1‑140 (MQ=255)                                                                                               tACGGAGGCGCTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGgttg                          <  2:488966/140‑1 (MQ=255)                                                                                                      gcgcTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGttttttt                   >  1:279538/1‑140 (MQ=255)                                                                                                      gcgcTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAAGCCGCGCTTTTGCTGCTCGCCGTGCTGTTGTCCTTCCATCATGAAAACTCGTGCGGGGGGTGttttttt                   >  2:457352/1‑140 (MQ=255)                                                                                                      gcgcTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTATCTGCTCGCCGTGCTGTTGGCCTTCCATCATGAAAACTCGTGCGGGGGGTGgtttttt                   >  2:445535/1‑140 (MQ=255)                                                                                                      gcgcTACCCAGGAATAACCCGGTTACTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCGAGCTTTAGCTGCTCCCCGTGCTGTTGGCCTTCCATCATGAAAACCCGTGCGGGGGGTGttttttt                   >  2:428620/1‑140 (MQ=255)                                                                                                         cTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGAt                <  1:141929/140‑1 (MQ=255)                                                                                                         cTACCCAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGAt                <  1:330984/140‑1 (MQ=255)                                                                                                             ccAGGAATAACCCGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTAc            <  1:457352/140‑1 (MQ=255)                                                                                                                       cccGGTTCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGc  <  1:329060/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                      CGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGC  >  NC_000913/121338‑121595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑