Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,900,948	T→C	Y213C (TAC→TGC)	yoaE ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,900,948	0	T	C	100.0%	117.3 / NA	33	Y213C (TAC→TGC)	yoaE	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/17);  total (16/17)

GCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAG  >  NC_000913/1900840‑1901078                                                                                                             |                                                                                                                                   gCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTc                                                                                                     >  1:161603/1‑140 (MQ=255) gCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTc                                                                                                     >  1:186373/1‑140 (MQ=255)     tACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTg                                                                                                   >  1:201833/1‑138 (MQ=255)     tACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTg                                                                                                   <  2:201833/138‑1 (MQ=255)     tACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGcc                                                                                                 >  2:488272/1‑140 (MQ=255)     tACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGcc                                                                                                 >  2:116471/1‑140 (MQ=255)      aCGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGcca                                                                                                >  1:101868/1‑140 (MQ=255)       cgcgCTCGCAGCGGCATAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGccac                                                                                               <  2:99089/140‑1 (MQ=255)        gcgcTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGccacc                                                                                              <  1:27942/140‑1 (MQ=255)               cAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTc                                                                                                     <  2:436813/126‑1 (MQ=255)               cAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTc                                                                                                     >  1:436813/1‑126 (MQ=255)                      aaaGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAAt                                                                                <  2:79317/140‑1 (MQ=255)                         gTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCAt                                                                             <  1:69123/140‑1 (MQ=255)                                    gCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAg                                                                       <  1:204882/135‑1 (MQ=255)                                    gCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAg                                                                       >  2:204882/1‑135 (MQ=255)                                         tAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcaga                                                             <  1:116471/140‑1 (MQ=255)                                            aGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcagacag                                                          <  1:406038/140‑1 (MQ=255)                                            aGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcagacag                                                          <  2:101868/140‑1 (MQ=255)                                                 cGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGcac                                                     >  1:143951/1‑140 (MQ=255)                                                      cgcAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGcaccacca                                                >  2:483427/1‑140 (MQ=255)                                                             tGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGAACAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTggg                                         <  2:143951/140‑1 (MQ=255)                                                                         tCGATGATGATCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAAc                             >  1:129229/1‑140 (MQ=255)                                                                                  atCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAAt                         >  2:189019/1‑135 (MQ=255)                                                                                  atCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAAt                         <  1:189019/135‑1 (MQ=255)                                                                                  atCGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGt                    >  1:160510/1‑140 (MQ=255)                                                                                    cGAGAAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCa                  >  2:493485/1‑140 (MQ=255)                                                                                     gagaAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAg                 <  2:160510/140‑1 (MQ=255)                                                                                     gagaAGCCAATCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAg                 <  1:488272/140‑1 (MQ=255)                                                                                               tCGCGGCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGAt       >  2:227494/1‑140 (MQ=255)                                                                                                   ggCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCa   <  1:366735/140‑1 (MQ=255)                                                                                                   ggCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCa   <  1:281934/140‑1 (MQ=255)                                                                                                    gCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAg  <  2:186373/140‑1 (MQ=255)                                                                                                    gCATACAGGCAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAg  >  1:400661/1‑140 (MQ=255)                                                                                                             |                                                                                                                                   GCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAG  >  NC_000913/1900840‑1901078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑