Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,949,990	A→G	V178A (GTG→GCG)	aspS ←	aspartate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,990	0	A	G	100.0%	53.9 / NA	16	V178A (GTG→GCG)	aspS	aspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/5);  total (11/5)

AAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGC  >  NC_000913/1949876‑1950120                                                                                                                   |                                                                                                                                   aaGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCgg                                                                                                           >  2:270942/1‑140 (MQ=255)   gCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCg                                                                                                         <  1:168238/140‑1 (MQ=255)                      tAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTTCCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCgg                                                                                      <  2:79389/140‑1 (MQ=255)                       aaCGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGa                                                                                     >  1:474669/1‑140 (MQ=255)                            tCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTc                                                                                >  1:514288/1‑140 (MQ=255)                            tCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTc                                                                                >  1:203044/1‑140 (MQ=255)                                     gACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAg                                                                       <  2:203044/140‑1 (MQ=255)                                     gACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAg                                                                       <  2:514288/140‑1 (MQ=255)                                         tcatcaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAg                                                                   <  2:63989/140‑1 (MQ=255)                                                 agcTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTACTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTc                                                           <  2:474669/140‑1 (MQ=255)                                                     gTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATcc                                                       >  2:110281/1‑140 (MQ=255)                                                       ttGAACAACTGCGGGGATTGCGGCAGTGCGTAGANTTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCAt                                                     >  1:134487/1‑140 (MQ=255)                                                             aacTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACg                                               >  2:32700/1‑140 (MQ=255)                                                                         tGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCt                                   >  1:67355/1‑140 (MQ=255)                                                                         tGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCt                                   >  2:118942/1‑140 (MQ=255)                                                                                                    tgCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGgcg        >  2:129827/1‑140 (MQ=255)                                                                                                         gcGAGAAGGCGCCAGGTAGTAACGCGCGACTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCAGTGGTCCTCCATAAAACGGCGCACCCGGCTGGTGATTCTAGCGCGGGTTTTCAGGCGCTggg   <  2:537883/139‑3 (MQ=255)                                                                                                            agaAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGc  >  2:264584/1‑138 (MQ=255)                                                                                                            agaAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGATTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGc  <  1:264584/138‑1 (MQ=255)                                                                                                                   |                                                                                                                                   AAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGC  >  NC_000913/1949876‑1950120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑