Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,965,961	G→A	I77I (ATC→ATT)	flhB ←	flagellar biosynthesis protein FlhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,965,961	0	G	A	100.0%	62.0 / NA	19	I77I (ATC→ATT)	flhB	flagellar biosynthesis protein FlhB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/10);  total (9/10)

AAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCA  >  NC_000913/1965833‑1966073                                                                                                                                 |                                                                                                                 aaGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCgg                                                                                                       <  1:541112/140‑1 (MQ=255)  aGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCggg                                                                                                      <  1:3089/140‑1 (MQ=255)    gATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTc                                                                                                    <  2:341296/140‑1 (MQ=255)                        cccTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCaa                                                                                >  1:420032/1‑140 (MQ=255)                                   tGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCa                                                                     <  2:420032/140‑1 (MQ=255)                                     aCCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGc                                                                   >  1:117426/1‑140 (MQ=255)                                     aCCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGc                                                                   >  2:98706/1‑140 (MQ=255)                                      ccGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCg                                                                  <  1:285265/140‑1 (MQ=255)                                      ccGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCg                                                                  >  2:88683/1‑140 (MQ=255)                                                        ccaGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAAt                                                >  1:367212/1‑140 (MQ=255)                                                                acGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGc                                        >  2:371188/1‑140 (MQ=255)                                                                         aTCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGacac                               <  2:117426/140‑1 (MQ=255)                                                                              cGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCACAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGc                          >  1:29541/1‑140 (MQ=255)                                                                                  agcagcGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCaaa                      <  1:368960/140‑1 (MQ=255)                                                                                  agcagcGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCaaa                      <  1:98706/140‑1 (MQ=255)                                                                                           aGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAac             >  1:28009/1‑140 (MQ=255)                                                                                             cATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCaaa                      >  2:468804/1‑129 (MQ=255)                                                                                             cATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCaaa                      <  1:468804/129‑1 (MQ=255)                                                                                                      tctGATCAGCAGAATAATCTGCCCGAGAATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCcca  <  1:30571/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                 AAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCA  >  NC_000913/1965833‑1966073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑